Down-regulation of type I protein kinase A by transfection of human breast cancer cells with an epidermal growth factor receptor antisense expression vector

Fortunato Ciardiello, Mniralini Dixit, Giuditta Di Isernia, Vincenzo Damiano, Roberto Bianco, A. Raffaele Bianco, Carlos L. Arteaga, Giampaolo Tortora

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

MDA-468 human breast cancer cells overexpress the EGFR and exhibit a functional TGFα-EGFR autocrine pathway. Loss of EGFR expression following stable transfection with an antisense EGFR cDNA containing plasmid down-regulates type T cAMP-dependent protein kinase (PKAI) expression with acquisition of cell growth resistance to the PKAI inhibitor 8-Cl-cAMP. These results suggest that PKAI expression and function are controlled by a TGFα-EGFR autocrine pathway in human breast cancer cells overexpressing the EGFR.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalBreast Cancer Research and Treatment
Volume47
Issue number1
DOIs
StatePublished - Feb 5 1998

Keywords

  • Antisense
  • Growth factors
  • Receptors

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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