TY - JOUR
T1 - Downregulation of the klotho gene in the kidney under sustained circulatory stress in rats
AU - Aizawa, Hiroki
AU - Saito, Yuichiro
AU - Nakamura, Tetsuya
AU - Inoue, Masahiro
AU - Imanari, Tetsuro
AU - Ohyama, Yoshio
AU - Matsumura, Yutaka
AU - Masuda, Hiroaki
AU - Oba, Shigenori
AU - Mise, Naobumi
AU - Kimura, Kenjiro
AU - Hasegawa, Akira
AU - Kurabayashi, Masahiko
AU - Kuro-O, Makoto
AU - Nabeshima, Yo Ichi
AU - Nagai, Ryozo
N1 - Funding Information:
We thank Yoshiko Nonaka, Mayumi Yoshii, Akemi Yoguchi, Shi-zuko Saiki, Miki Yamazaki, and Kaori Ishihara for their technical assistance. This study was supported by the Japanese Ministry of Education, Science and Culture, and the Promotion of Fundamental Studies in Health Science of the Organization for Pharmaceutical Safety and Research (OPSR).
PY - 1998/8/28
Y1 - 1998/8/28
N2 - We recently reported the isolation of the klotho gene, which is predominantly expressed in the kidney and involved in human aging phenotypes. In our previous studies, we demonstrated that the Klotho protein or its metabolites may possibly function as humoral factor(s) and protect against endothelial dysfunction because acetylcholine-mediated NO production in arteries was impaired in heterozygous klotho deficient mice (kl/+). However, the pathophysiological significance of the Klotho protein has not been clarified yet. In the present study, we examined expression of the klotho gene in the kidney of the following rat models for human diseases: (1) spontaneously hypertensive rat, (2) deoxycorticosterone acetate-salt hypertensive rat, (3) 5/6 nephrectomized rat, (4) non-insulin-dependent diabetes mellitus rat (the Otsuka Long-Evans Tokushima Fatty rat), and (5) rat with acute myocardial infarction. The expression levels of klotho mRNA in the kidney in these models were significantly lower than controls except for MI rats. This is the first report showing that expression of the klotho gene in the kidney is regulated under sustained circulatory stress such as long-term hypertension, diabetes mellitus, and chronic renal failure.
AB - We recently reported the isolation of the klotho gene, which is predominantly expressed in the kidney and involved in human aging phenotypes. In our previous studies, we demonstrated that the Klotho protein or its metabolites may possibly function as humoral factor(s) and protect against endothelial dysfunction because acetylcholine-mediated NO production in arteries was impaired in heterozygous klotho deficient mice (kl/+). However, the pathophysiological significance of the Klotho protein has not been clarified yet. In the present study, we examined expression of the klotho gene in the kidney of the following rat models for human diseases: (1) spontaneously hypertensive rat, (2) deoxycorticosterone acetate-salt hypertensive rat, (3) 5/6 nephrectomized rat, (4) non-insulin-dependent diabetes mellitus rat (the Otsuka Long-Evans Tokushima Fatty rat), and (5) rat with acute myocardial infarction. The expression levels of klotho mRNA in the kidney in these models were significantly lower than controls except for MI rats. This is the first report showing that expression of the klotho gene in the kidney is regulated under sustained circulatory stress such as long-term hypertension, diabetes mellitus, and chronic renal failure.
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U2 - 10.1006/bbrc.1998.9246
DO - 10.1006/bbrc.1998.9246
M3 - Article
C2 - 9731228
AN - SCOPUS:0032575483
SN - 0006-291X
VL - 249
SP - 865
EP - 871
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -