DQA1*03 subtypes have different associations with DRB1 and DQB1 alleles

Marcelo A. Fernandez-Viña, Michela Falco, Xiaojian Gao, Marie Cerna, Yiping Sun, Eduardo Raimondi, Peter Stastny

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Abstract

Polymorphisms outside the hypervariable regions of HLA class II alleles that do not affect the peptide-binding site are probably not under selective pressure and could therefore be useful as markers of the evolutionary pathways of the HLA class II haplotypes. We have analyzed such a polymorphism in the variants of DQA1*03, which differ at residue 160 encoded in exon 3. Our study included homozygous BCLs of the 10th IHWS and samples of a multiracial panel of 723 unrelated subjects which were also typed for allelic variations in exon 2 by hybridization with SSOP. BCLs having DQA1*03 and 131 selected DQA1*03-positive samples were typed for the dimorphism in exon 3 that distinguishes DQA1*0301 and DQA1*0302. DQA1*0301 was found to be exclusively associated with DQB1*0302, while samples carrying DQB1*0201, 0301, 0303, and 0401 always had DQA1*0302. A few haplotypes carrying DQB1*0302 had DQA1*0302. The fact that DQA1*0301 is completely included in DQB1*0302, and not vice versa, suggests that DQA1*0301 may have arisen from a mutation in a haplotype containing DQA1*0302-DQB1*0302. DQB1*0302 was found to be associated with all DR4 subtypes, suggesting possibly that the current variants of DRB1-DR4 may be of more recent origin. DRB1*0405 was the only subtype of DR4 which was not associated with DQA1*0301 and had multiple associations with the DQB1 alleles, therefore, perhaps representing the oldest allele of this group.

Original languageEnglish (US)
Pages (from-to)290-298
Number of pages9
JournalHuman Immunology
Volume39
Issue number4
DOIs
StatePublished - Apr 1994

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Fernandez-Viña, M. A., Falco, M., Gao, X., Cerna, M., Sun, Y., Raimondi, E., & Stastny, P. (1994). DQA1*03 subtypes have different associations with DRB1 and DQB1 alleles. Human Immunology, 39(4), 290-298. https://doi.org/10.1016/0198-8859(94)90272-0