DRA0336, another OxyR homolog, involved in the antioxidation mechanisms in Deinococcus radiodurans

Longfei Yin, Liangyan Wang, Huiming Lu, Guangzhi Xu, Huan Chen, Hongdan Zhan, Bing Tian, Yuejin Hua

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A novel OxyR (DR0615) with one conserved cysteine that senses hydrogen peroxide in Deinococcus radiodurans had been identified in our previous work. Comparative genomics revealed that D. radiodurans possesses another OxyR homolog, OxyR2 (DRA0336). In this study, we constructed the deletion mutant of oxyR2 and the double mutant of both the OxyR homologs to investigate the role of OxyR in response to oxidative stress in D. Radiodurans. Deletion of oxyR2 resulted in an obviously increased sensitivity to hydrogen peroxide, and the double mutant for oxyR and oxyR2 was significantly more sensitive than any of the two single mutants. The total catalase activity of the double mutant was lower than that of any of the single mutants, and reactive oxygen species (ROS) accumulated to a greater extent. DNA microarray analysis further suggested that oxyR2 was involved in antioxidation mechanisms. Site-direct mutagenesis and complementation analysis revealed that C228 in OxyR2 was essential. This is the first report of the presence of two OxyR in one organism. These results suggest that D. radiodurans OxyR and OxyR2 function together to protect the cell against oxidative stress.

Original languageEnglish (US)
Pages (from-to)473-479
Number of pages7
JournalJournal of Microbiology
Volume48
Issue number4
DOIs
StatePublished - Sep 6 2010

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Deinococcus
Hydrogen Peroxide
Oxidative Stress
Microarray Analysis
Genomics
Oligonucleotide Array Sequence Analysis
Mutagenesis
Catalase
Cysteine
Reactive Oxygen Species

Keywords

  • antioxidation mechanism
  • Deinococcus
  • oxidative stress
  • OxyR
  • OxyR

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

DRA0336, another OxyR homolog, involved in the antioxidation mechanisms in Deinococcus radiodurans. / Yin, Longfei; Wang, Liangyan; Lu, Huiming; Xu, Guangzhi; Chen, Huan; Zhan, Hongdan; Tian, Bing; Hua, Yuejin.

In: Journal of Microbiology, Vol. 48, No. 4, 06.09.2010, p. 473-479.

Research output: Contribution to journalArticle

Yin, Longfei ; Wang, Liangyan ; Lu, Huiming ; Xu, Guangzhi ; Chen, Huan ; Zhan, Hongdan ; Tian, Bing ; Hua, Yuejin. / DRA0336, another OxyR homolog, involved in the antioxidation mechanisms in Deinococcus radiodurans. In: Journal of Microbiology. 2010 ; Vol. 48, No. 4. pp. 473-479.
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