Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes

Richard D. Palmiter, Ralph L. Brinster, Robert E Hammer, Myrna E. Trumbauer, Michael G. Rosenfeld, Neal C. Birnberg, Ronald M. Evans

Research output: Contribution to journalArticle

821 Citations (Scopus)

Abstract

A DNA fragment containing the promoter of the mouse metallothionein-I gene fused to the structural gene of rat growth hormone was microinjected into the pronuclei of fertilized mouse eggs. Of 21 mice that developed from these eggs, seven carried the fusion gene and six of these grew significantly larger than their littermates. Several of these transgenic mice had extraordinarily high levels of the fusion mRNA in their liver and growth hormone in their serum. This approach has implications for studying the biological effects of growth hormone, as a way to accelerate animal growth, as a model for gigantism, as a means of correcting genetic disease, and as a method of farming valuable gene products.

Original languageEnglish (US)
Pages (from-to)611-615
Number of pages5
JournalNature
Volume300
Issue number5893
DOIs
StatePublished - 1982

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Metallothionein
Gene Fusion
Eggs
Growth Hormone
Growth
Gigantism
Genes
Inborn Genetic Diseases
Zygote
Agriculture
Transgenic Mice
Messenger RNA
Liver
DNA
Serum

ASJC Scopus subject areas

  • General

Cite this

Palmiter, R. D., Brinster, R. L., Hammer, R. E., Trumbauer, M. E., Rosenfeld, M. G., Birnberg, N. C., & Evans, R. M. (1982). Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature, 300(5893), 611-615. https://doi.org/10.1038/300611a0

Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. / Palmiter, Richard D.; Brinster, Ralph L.; Hammer, Robert E; Trumbauer, Myrna E.; Rosenfeld, Michael G.; Birnberg, Neal C.; Evans, Ronald M.

In: Nature, Vol. 300, No. 5893, 1982, p. 611-615.

Research output: Contribution to journalArticle

Palmiter, RD, Brinster, RL, Hammer, RE, Trumbauer, ME, Rosenfeld, MG, Birnberg, NC & Evans, RM 1982, 'Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes', Nature, vol. 300, no. 5893, pp. 611-615. https://doi.org/10.1038/300611a0
Palmiter, Richard D. ; Brinster, Ralph L. ; Hammer, Robert E ; Trumbauer, Myrna E. ; Rosenfeld, Michael G. ; Birnberg, Neal C. ; Evans, Ronald M. / Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. In: Nature. 1982 ; Vol. 300, No. 5893. pp. 611-615.
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