Dredd, a novel effector of the apoptosis activators Reaper, Grim, and Hid in Drosophila

Po Chen, Antony Rodriguez, Robert Erskine, Tien Thach, John M. Abrams

Research output: Contribution to journalArticlepeer-review

176 Scopus citations

Abstract

Caspases are widely conserved proteases considered to be essential effectors of apoptosis. We identified a novel Drosophila gene, dredd, which shares extensive homology to all members of the caspase gene family. Cells specified for programmed death in development exhibit a striking accumulation of dredd RNA that requires signaling by the death activators REAPER, GRIM, and HID. Furthermore, directed misexpression of each activator was sufficient to drive ectopic accumulation of dredd RNA. Heterozygosity at the dredd locus suppressed apoptosis in transgenic models of reaper- and grim-induced cell killing, demonstrating that levels of dredd product can modulate signaling triggered by these death activators. Finally, expression of REAPER, GRIM, and HID was found to trigger processing of DREDD protein precursor through a mechanism that is insensitive to, and upstream of, known caspase inhibitors. Taken together, these observations establish mechanistic connections between activators of apoptosis and a new downstream death effector in Drosophila.

Original languageEnglish (US)
Pages (from-to)202-216
Number of pages15
JournalDevelopmental Biology
Volume201
Issue number2
DOIs
StatePublished - Sep 15 1998

Keywords

  • Apoptosis
  • Caspase
  • Drosophila
  • Programmed cell death

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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