Drifting motions of the adenovirus receptor CAR and immobile integrins initiate virus uncoating and membrane lytic protein exposure

Christoph J. Burckhardt, Maarit Suomalainen, Philipp Schoenenberger, Karin Boucke, Silvio Hemmi, Urs F. Greber

Research output: Contribution to journalArticlepeer-review

119 Scopus citations

Abstract

Viral particle binding to plasma membrane receptors elicits virus motions, recruits signaling proteins, and triggers membrane bending and fission, finally resulting in endocytic virus uptake. Here we analyze how human adenovirus engages its receptor coxsackievirus adenovirus receptor (CAR) and coreceptor αv integrin to move on the plasma membrane. Virus binding to CAR through fiber knobs gave rise to diffusive motions and actomyosin-2-dependent drifts, while integrin-targeted viruses were spatially more confined. Diffusions, drifts, and confined motions were specifically observed with viral particles that were subsequently internalized. CAR-mediated drifts together with integrin binding supported fiber shedding from adenovirus particles, leading to exposure of the membrane-lytic internal virion protein VI and enhanced viral escape from endosomes. Our results show that adenovirus uncoating is initiated at the plasma membrane by CAR drifting motion and binding to immobile integrins.

Original languageEnglish (US)
Pages (from-to)105-117
Number of pages13
JournalCell Host and Microbe
Volume10
Issue number2
DOIs
StatePublished - Aug 18 2011

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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