Drosophila acinus encodes a novel regulator of endocytic and autophagic trafficking

Adam S. Haberman, Mohammed Ali Akbar, Sanchali Ray, Helmut Krämer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Endosomal trafficking affects many cellular pathways from cell signaling to metabolism, but little is known about how these effects are coordinated. In a genetic screen for mutants affecting endosomal trafficking, we identified Drosophila acinus (dacn; hook-like). Its mammalian homolog Acinus has been implicated in RNA processing and chromatin fragmentation during apoptosis. Loss-of-function analysis of dacn revealed two distinct functions. First, dacn is required for stabilization of early endosomes, thus modulating levels of Notch and Egfr signaling. Second, loss of dacn interferes with cellular starvation responses by inhibiting autophagosome maturation. By contrast, overexpression of dacn causes lethality due to enhanced autophagy. We show that this enhanced autophagy is independent of the Tor pathway. Taken together, our data show that dacn encodes a regulator of endosomal and autophagosomal dynamics, modulating developmental signaling and the cellular response to starvation.

Original languageEnglish (US)
Pages (from-to)2157-2166
Number of pages10
JournalDevelopment
Volume137
Issue number13
DOIs
StatePublished - Jul 1 2010

Fingerprint

Autophagy
Starvation
Drosophila
Endosomes
Chromatin
RNA
Apoptosis
Autophagosomes

Keywords

  • Acinus
  • Autophagosomes
  • Drosophila
  • Egfr
  • Endocytic trafficking
  • Notch

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology

Cite this

Drosophila acinus encodes a novel regulator of endocytic and autophagic trafficking. / Haberman, Adam S.; Akbar, Mohammed Ali; Ray, Sanchali; Krämer, Helmut.

In: Development, Vol. 137, No. 13, 01.07.2010, p. 2157-2166.

Research output: Contribution to journalArticle

Haberman, Adam S. ; Akbar, Mohammed Ali ; Ray, Sanchali ; Krämer, Helmut. / Drosophila acinus encodes a novel regulator of endocytic and autophagic trafficking. In: Development. 2010 ; Vol. 137, No. 13. pp. 2157-2166.
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