Drosophila CG2469 encodes a homolog of human CTR9 and is essential for development

Dhananjay Chaturvedi, Mayu Inaba, Shane Scoggin, Michael Buszczak

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Conserved from yeast to humans, the Paf1 complex participates in a number of diverse processes including transcriptional initiation and polyadenylation. This complex typically includes five proteins: Paf1, Rtf1, Cdc73, Leo1, and Ctr9. Previous efforts identified clear Drosophila homologs of Paf1, Rtf1, and Cdc73 based on sequence similarity. Further work showed that these proteins help to regulate gene expression and are required for viability. To date, a Drosophila homolog of Ctr9 has remained uncharacterized. Here, we show that the gene CG2469 encodes a functional Drosophila Ctr9 homolog. Both human and Drosophila Ctr9 localize to the nuclei of Drosophila cells and appear enriched in histone locus bodies. RNAi knockdown of Drosophila Ctr9 results in a germline stem cell loss phenotype marked by defects in the morphology of germ cell nuclei. A molecular null mutation of Drosophila Ctr9 results in lethality and a human cDNA CTR9 transgene rescues this phenotype. Clonal analysis in the ovary using this null allele reveals that loss of Drosophila Ctr9 results in a reduction of global levels of histone H3 trimethylation of lysine 4 (H3K4me3), but does not compromise the maintenance of stem cells in ovaries. Given the differences between the null mutant and RNAi knockdown phenotypes, the germ cell defects caused by RNAi likely result from the combined loss of Drosophila Ctr9 and other unidentified genes. These data provide further evidence that the function of this Paf1 complex component is conserved across species.

Original languageEnglish (US)
Pages (from-to)3849-3857
Number of pages9
JournalG3: Genes, Genomes, Genetics
Volume6
Issue number12
DOIs
StatePublished - 2016

Fingerprint

Drosophila
RNA Interference
Cell Nucleus
Phenotype
Germ Cells
Histones
Ovary
Stem Cells
Polyadenylation
Transgenes
Genes
Lysine
Proteins
Complementary DNA
Yeasts
Alleles
Maintenance
Gene Expression
Mutation

Keywords

  • Chromatin
  • Ctr9
  • Germline
  • PAF1 complex
  • RNAi

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Drosophila CG2469 encodes a homolog of human CTR9 and is essential for development. / Chaturvedi, Dhananjay; Inaba, Mayu; Scoggin, Shane; Buszczak, Michael.

In: G3: Genes, Genomes, Genetics, Vol. 6, No. 12, 2016, p. 3849-3857.

Research output: Contribution to journalArticle

Chaturvedi, Dhananjay ; Inaba, Mayu ; Scoggin, Shane ; Buszczak, Michael. / Drosophila CG2469 encodes a homolog of human CTR9 and is essential for development. In: G3: Genes, Genomes, Genetics. 2016 ; Vol. 6, No. 12. pp. 3849-3857.
@article{ab0408bb841a45d78d91ed8a2eca426c,
title = "Drosophila CG2469 encodes a homolog of human CTR9 and is essential for development",
abstract = "Conserved from yeast to humans, the Paf1 complex participates in a number of diverse processes including transcriptional initiation and polyadenylation. This complex typically includes five proteins: Paf1, Rtf1, Cdc73, Leo1, and Ctr9. Previous efforts identified clear Drosophila homologs of Paf1, Rtf1, and Cdc73 based on sequence similarity. Further work showed that these proteins help to regulate gene expression and are required for viability. To date, a Drosophila homolog of Ctr9 has remained uncharacterized. Here, we show that the gene CG2469 encodes a functional Drosophila Ctr9 homolog. Both human and Drosophila Ctr9 localize to the nuclei of Drosophila cells and appear enriched in histone locus bodies. RNAi knockdown of Drosophila Ctr9 results in a germline stem cell loss phenotype marked by defects in the morphology of germ cell nuclei. A molecular null mutation of Drosophila Ctr9 results in lethality and a human cDNA CTR9 transgene rescues this phenotype. Clonal analysis in the ovary using this null allele reveals that loss of Drosophila Ctr9 results in a reduction of global levels of histone H3 trimethylation of lysine 4 (H3K4me3), but does not compromise the maintenance of stem cells in ovaries. Given the differences between the null mutant and RNAi knockdown phenotypes, the germ cell defects caused by RNAi likely result from the combined loss of Drosophila Ctr9 and other unidentified genes. These data provide further evidence that the function of this Paf1 complex component is conserved across species.",
keywords = "Chromatin, Ctr9, Germline, PAF1 complex, RNAi",
author = "Dhananjay Chaturvedi and Mayu Inaba and Shane Scoggin and Michael Buszczak",
year = "2016",
doi = "10.1534/g3.116.035196",
language = "English (US)",
volume = "6",
pages = "3849--3857",
journal = "G3 (Bethesda, Md.)",
issn = "2160-1836",
publisher = "Genetics Society of America",
number = "12",

}

TY - JOUR

T1 - Drosophila CG2469 encodes a homolog of human CTR9 and is essential for development

AU - Chaturvedi, Dhananjay

AU - Inaba, Mayu

AU - Scoggin, Shane

AU - Buszczak, Michael

PY - 2016

Y1 - 2016

N2 - Conserved from yeast to humans, the Paf1 complex participates in a number of diverse processes including transcriptional initiation and polyadenylation. This complex typically includes five proteins: Paf1, Rtf1, Cdc73, Leo1, and Ctr9. Previous efforts identified clear Drosophila homologs of Paf1, Rtf1, and Cdc73 based on sequence similarity. Further work showed that these proteins help to regulate gene expression and are required for viability. To date, a Drosophila homolog of Ctr9 has remained uncharacterized. Here, we show that the gene CG2469 encodes a functional Drosophila Ctr9 homolog. Both human and Drosophila Ctr9 localize to the nuclei of Drosophila cells and appear enriched in histone locus bodies. RNAi knockdown of Drosophila Ctr9 results in a germline stem cell loss phenotype marked by defects in the morphology of germ cell nuclei. A molecular null mutation of Drosophila Ctr9 results in lethality and a human cDNA CTR9 transgene rescues this phenotype. Clonal analysis in the ovary using this null allele reveals that loss of Drosophila Ctr9 results in a reduction of global levels of histone H3 trimethylation of lysine 4 (H3K4me3), but does not compromise the maintenance of stem cells in ovaries. Given the differences between the null mutant and RNAi knockdown phenotypes, the germ cell defects caused by RNAi likely result from the combined loss of Drosophila Ctr9 and other unidentified genes. These data provide further evidence that the function of this Paf1 complex component is conserved across species.

AB - Conserved from yeast to humans, the Paf1 complex participates in a number of diverse processes including transcriptional initiation and polyadenylation. This complex typically includes five proteins: Paf1, Rtf1, Cdc73, Leo1, and Ctr9. Previous efforts identified clear Drosophila homologs of Paf1, Rtf1, and Cdc73 based on sequence similarity. Further work showed that these proteins help to regulate gene expression and are required for viability. To date, a Drosophila homolog of Ctr9 has remained uncharacterized. Here, we show that the gene CG2469 encodes a functional Drosophila Ctr9 homolog. Both human and Drosophila Ctr9 localize to the nuclei of Drosophila cells and appear enriched in histone locus bodies. RNAi knockdown of Drosophila Ctr9 results in a germline stem cell loss phenotype marked by defects in the morphology of germ cell nuclei. A molecular null mutation of Drosophila Ctr9 results in lethality and a human cDNA CTR9 transgene rescues this phenotype. Clonal analysis in the ovary using this null allele reveals that loss of Drosophila Ctr9 results in a reduction of global levels of histone H3 trimethylation of lysine 4 (H3K4me3), but does not compromise the maintenance of stem cells in ovaries. Given the differences between the null mutant and RNAi knockdown phenotypes, the germ cell defects caused by RNAi likely result from the combined loss of Drosophila Ctr9 and other unidentified genes. These data provide further evidence that the function of this Paf1 complex component is conserved across species.

KW - Chromatin

KW - Ctr9

KW - Germline

KW - PAF1 complex

KW - RNAi

UR - http://www.scopus.com/inward/record.url?scp=85008506880&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008506880&partnerID=8YFLogxK

U2 - 10.1534/g3.116.035196

DO - 10.1534/g3.116.035196

M3 - Article

VL - 6

SP - 3849

EP - 3857

JO - G3 (Bethesda, Md.)

JF - G3 (Bethesda, Md.)

SN - 2160-1836

IS - 12

ER -