Drosophila p53 preserves genomic stability by regulating cell death

Naoko Sogame, Misoo Kim, John M. Abrams

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

When animal cells are exposed to stressful conditions, the tumor suppressor protein p53 restrains growth by promoting an arrested cell cycle or initiating a cell death program. How these distinct fates are specified through the action of a single protein is not known. To study its functions in vivo we produced a targeted mutation at the Drosophila p53 (Dmp53) locus. We show that Dmp53 is required for damage-induced apoptosis but not for cell-cycle arrest. Dmp53 function is also required for damage-induced transcription of two tightly linked cell death activators, reaper and sickle. When challenged by ionizing radiation, Dmp53 mutants exhibit radiosensitivity and genomic instability. Hence, elevated mutant loads were not caused by defective checkpoint functions but instead correlated with failures in p53-associated cell death. Our studies support the notion that core ancestral functions of the p53 gene family are intimately coupled to cell death as an adaptive response to maintain genomic stability.

Original languageEnglish (US)
Pages (from-to)4696-4701
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number8
DOIs
StatePublished - Apr 15 2003

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Genomic Instability
Drosophila
Cell Death
Tumor Suppressor Protein p53
Radiation Tolerance
p53 Genes
Cell Cycle Checkpoints
Ionizing Radiation
Cell Cycle
Apoptosis
Mutation
Growth
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Drosophila p53 preserves genomic stability by regulating cell death. / Sogame, Naoko; Kim, Misoo; Abrams, John M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 8, 15.04.2003, p. 4696-4701.

Research output: Contribution to journalArticle

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