Drosophila Rrpl complements E.coli xth nfo mutants

Protection against both oxidative and alkylation-induced DNA damage

Liya Gu, Shu mei Huang, Miriam Sander

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Drosophila, Rrp1 protein has four tightly associated enzymatic activities: DNA strand transfer, ssDNA renaturation, dsDNA 3′-exonuclease and apurinic/apyrimidinic (AP) endonuclease. The carboxy-terminal region of Rrp1 is homologous to Escherichia coli exonuclease III and several eukaryotic AP endonucleases. All members of this protein family cleave abasic sites. Rrp1 protein was expressed under the control of the E.coli RNA polymerase tac promoter (pRrp1-tac) in two repair deficientE.coli strains (BW528 and LG101) lacking both exonuclease III (xth) and endonuclease IV (nfo). Rrp1 confers resistance to killing by oxidative, antitumor and alkylating agents that damage DNA (hydrogen peroxide, t-butylhydroperoxide, bleomycin, methyl methanesulfonate, and mitomycin C). Complementation of the repair deficiency by Rrp1 provides up to a two log increase in survival and requires the C-terminal nuclease region of Rrp1, but not its N-terminal region. The AP endonuclease activity in extracts from the repair deficient strain LG101 is increased up to 12-fold when the strain contains pRrp1-tac. These results indicate that pRrp1-tac directs the synthesis of active enzyme, and that the nuclease activities of Rrp1 are likely to be the cause of the increased resistance to DNA damage of the mutant cells.

Original languageEnglish (US)
Pages (from-to)4788-4795
Number of pages8
JournalNucleic Acids Research
Volume21
Issue number20
DOIs
StatePublished - Oct 11 1993

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spleen exonuclease
Endonucleases
Alkylation
DNA Damage
Drosophila
Deoxyribonuclease IV (Phage T4-Induced)
DNA-(Apurinic or Apyrimidinic Site) Lyase
Escherichia coli
Methyl Methanesulfonate
tert-Butylhydroperoxide
Alkylating Agents
Bleomycin
Mitomycin
DNA-Directed RNA Polymerases
Antineoplastic Agents
Hydrogen Peroxide
Proteins
DNA
Enzymes
exodeoxyribonuclease III

ASJC Scopus subject areas

  • Genetics

Cite this

Drosophila Rrpl complements E.coli xth nfo mutants : Protection against both oxidative and alkylation-induced DNA damage. / Gu, Liya; Huang, Shu mei; Sander, Miriam.

In: Nucleic Acids Research, Vol. 21, No. 20, 11.10.1993, p. 4788-4795.

Research output: Contribution to journalArticle

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