Drosophila stem cells share a common requirement for the histone H2B ubiquitin protease scrawny

Michael Buszczak, Shelley Paterno, Allan C. Spradling

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Stem cells within diverse tissues share the need for a chromatin configuration that promotes self-renewal, yet few chromatin proteins are known to regulate multiple types of stem cells. We describe a Drosophila gene, scrawny (scny), encoding a ubiquitin-specific protease, which is required in germline, epithelial, and intestinal stem cells. Like its yeast relative UBP10, Scrawny deubiquitylates histone H2B and functions in gene silencing. Consistent with previous studies of this conserved pathway of chromatin regulation, scny mutant cells have elevated levels of ubiquitinylated H2B and trimethylated H3K4. Our findings suggest that inhibiting H2B ubiquitylation through scny represents a common mechanism within stem cells that is used to repress the premature expression of key differentiation genes, including Notch target genes.

Original languageEnglish (US)
Pages (from-to)248-251
Number of pages4
JournalScience
Volume323
Issue number5911
DOIs
StatePublished - Jan 9 2009

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Ubiquitin
Drosophila
Stem Cells
Chromatin
Ubiquitin-Specific Proteases
Genes
Ubiquitination
Gene Silencing
Histones
Yeasts
histone proteases
Proteins

ASJC Scopus subject areas

  • General

Cite this

Drosophila stem cells share a common requirement for the histone H2B ubiquitin protease scrawny. / Buszczak, Michael; Paterno, Shelley; Spradling, Allan C.

In: Science, Vol. 323, No. 5911, 09.01.2009, p. 248-251.

Research output: Contribution to journalArticle

Buszczak, Michael ; Paterno, Shelley ; Spradling, Allan C. / Drosophila stem cells share a common requirement for the histone H2B ubiquitin protease scrawny. In: Science. 2009 ; Vol. 323, No. 5911. pp. 248-251.
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