TY - JOUR
T1 - Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner
AU - Pennetta, Giuseppa
AU - Hiesinger, Peter Robin
AU - Fabian-Fine, Ruth
AU - Meinertzhagen, Ian A.
AU - Bellen, Hugo J.
N1 - Funding Information:
We thank L. Zipursky, N. Harden, C. Goodman, P. Bryant, and V. Budnik for antibodies. We also thank D. Greenstein, V. Budnik, and A. Fayyazuddin for critical reading. We thank J. Kunz for stimulating discussions and Y. Zhou for technical assistance. G.P. is supported by HHMI, P.R.H. by EMBO, R.F.F. by the Killam Trust of Dalhousie University, and I.A.M. by NIH grant EY-03592 and by the Killam Trust of Dalhousie University. H.J.B. is an Investigator of the HHMI.
PY - 2002/7/18
Y1 - 2002/7/18
N2 - Aplysia VAP-33 (VAMP-associated protein) has been previously proposed to be involved in the control of neurotransmitter release. Here, we show that a Drosophila homolog of VAP-33, DVAP-33A, is localized to neuromuscular junctions. Loss of DVAP-33A causes a severe decrease in the number of boutons and a corresponding increase in bouton size. Conversely, presynaptic overexpression of DVAP-33A induces an increase in the number of boutons and a decrease in their size. Gain-of-function experiments show that the presynaptic dose of DVAP-33A tightly modulates the number of synaptic boutons. Our data also indicate that the presynaptic microtubule architecture is severely compromised in DVAP-33A mutants. We propose that a DVAP-33A-mediated interaction between microtubules and presynaptic membrane plays a pivotal role during bouton budding.
AB - Aplysia VAP-33 (VAMP-associated protein) has been previously proposed to be involved in the control of neurotransmitter release. Here, we show that a Drosophila homolog of VAP-33, DVAP-33A, is localized to neuromuscular junctions. Loss of DVAP-33A causes a severe decrease in the number of boutons and a corresponding increase in bouton size. Conversely, presynaptic overexpression of DVAP-33A induces an increase in the number of boutons and a decrease in their size. Gain-of-function experiments show that the presynaptic dose of DVAP-33A tightly modulates the number of synaptic boutons. Our data also indicate that the presynaptic microtubule architecture is severely compromised in DVAP-33A mutants. We propose that a DVAP-33A-mediated interaction between microtubules and presynaptic membrane plays a pivotal role during bouton budding.
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U2 - 10.1016/S0896-6273(02)00769-9
DO - 10.1016/S0896-6273(02)00769-9
M3 - Article
C2 - 12160747
AN - SCOPUS:0037130456
SN - 0896-6273
VL - 35
SP - 291
EP - 306
JO - Neuron
JF - Neuron
IS - 2
ER -