Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner

Giuseppa Pennetta, Peter Robin Hiesinger, Ruth Fabian-Fine, Ian A. Meinertzhagen, Hugo J. Bellen

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Aplysia VAP-33 (VAMP-associated protein) has been previously proposed to be involved in the control of neurotransmitter release. Here, we show that a Drosophila homolog of VAP-33, DVAP-33A, is localized to neuromuscular junctions. Loss of DVAP-33A causes a severe decrease in the number of boutons and a corresponding increase in bouton size. Conversely, presynaptic overexpression of DVAP-33A induces an increase in the number of boutons and a decrease in their size. Gain-of-function experiments show that the presynaptic dose of DVAP-33A tightly modulates the number of synaptic boutons. Our data also indicate that the presynaptic microtubule architecture is severely compromised in DVAP-33A mutants. We propose that a DVAP-33A-mediated interaction between microtubules and presynaptic membrane plays a pivotal role during bouton budding.

Original languageEnglish (US)
Pages (from-to)291-306
Number of pages16
JournalNeuron
Volume35
Issue number2
DOIs
StatePublished - Jul 18 2002

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Neuromuscular Junction
Microtubules
Drosophila
Aplysia
Presynaptic Terminals
Neurotransmitter Agents
Membranes
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pennetta, G., Hiesinger, P. R., Fabian-Fine, R., Meinertzhagen, I. A., & Bellen, H. J. (2002). Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner. Neuron, 35(2), 291-306. https://doi.org/10.1016/S0896-6273(02)00769-9

Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner. / Pennetta, Giuseppa; Hiesinger, Peter Robin; Fabian-Fine, Ruth; Meinertzhagen, Ian A.; Bellen, Hugo J.

In: Neuron, Vol. 35, No. 2, 18.07.2002, p. 291-306.

Research output: Contribution to journalArticle

Pennetta, G, Hiesinger, PR, Fabian-Fine, R, Meinertzhagen, IA & Bellen, HJ 2002, 'Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner', Neuron, vol. 35, no. 2, pp. 291-306. https://doi.org/10.1016/S0896-6273(02)00769-9
Pennetta G, Hiesinger PR, Fabian-Fine R, Meinertzhagen IA, Bellen HJ. Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner. Neuron. 2002 Jul 18;35(2):291-306. https://doi.org/10.1016/S0896-6273(02)00769-9
Pennetta, Giuseppa ; Hiesinger, Peter Robin ; Fabian-Fine, Ruth ; Meinertzhagen, Ian A. ; Bellen, Hugo J. / Drosophila VAP-33A directs bouton formation at neuromuscular junctions in a dosage-dependent manner. In: Neuron. 2002 ; Vol. 35, No. 2. pp. 291-306.
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