Db plus peptide protective motifs prevent rejection of D8P2+ but not Dd+ bone marrow cells(BMC) by B6 host natural killer(NK) cells

J. Liu, C. Sentman, K. Fischer Lindahl, V. Kumar, M. Bennett

Research output: Contribution to journalArticle

Abstract

Crosses between wild type spretus and BIO H2b mice produced congenic B10.SP2 and intra-H2 recombinant 9347 and R40 mice. 9347 and R40 mice are KISb/DM3sp2 (breakpoints between Bat2-Tnf segments of S/D interval). Bone marrow cells of B 10.SP2, 9347 and R40 mice were rejected by B6 H2b host NK cells as if B6 NK cells recognize codominantly inherited Dsp2 on donor BMC. 9347 × B6 F1 but not R40 X B6 BMC were accepted by B6 hosts, suggesting that Db plus a Bat2-Tnfb peptide from 9347 but not of R40 form a protective motif to prevent rejection by B6 host. Therefore 9347 and R40 mice were crossed to DBA.Db(H2dDb) transgenic mice to produce Kb/dDsp2/dLd, Db F1 mice; their BMC cells were rejected by B6 but were accepted by D8(H2bDd) hosts as if Dd was the target BMC from F2 donors that were H29347+(or even H2R40+), H2d-, Db+ grew in B6 hosts; BMC from Db- donors were rejected. Db plus peptides from non-H2d DBA genes may generate protective motifs for Dsp2 but not for Dd targets. Thus specific peptides and class I molecules are apparently required to create negative signaling motifs for NK cells to prevent rejection of BMC. BMC Donor 5-Day 125IUdR Uptake in Spleens Strain K D L Hosts: B6 D8: H2b, Dd R40 or 9347 b sp2 B6 × R40 b b/sp2 - NT B6 × 9347 b Wsp2 + NT 9347 × DBA.Db F1 b/d Sp2/d,b d - + 9347 × DBA.Db F2 b sp2, b+ + NT 9347 × DBA.Db F2 b sp2, b- - NT.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

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Allura Red AC Dye
bone marrow cells
natural killer cells
Natural Killer Cells
Bone Marrow Cells
Bone
Cells
peptides
Peptides
mice
Congenic Mice
Transgenic Mice
spleen
Spleen
Genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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Db plus peptide protective motifs prevent rejection of D8P2+ but not Dd+ bone marrow cells(BMC) by B6 host natural killer(NK) cells. / Liu, J.; Sentman, C.; Fischer Lindahl, K.; Kumar, V.; Bennett, M.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Liu, J. ; Sentman, C. ; Fischer Lindahl, K. ; Kumar, V. ; Bennett, M. / Db plus peptide protective motifs prevent rejection of D8P2+ but not Dd+ bone marrow cells(BMC) by B6 host natural killer(NK) cells. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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abstract = "Crosses between wild type spretus and BIO H2b mice produced congenic B10.SP2 and intra-H2 recombinant 9347 and R40 mice. 9347 and R40 mice are KISb/DM3sp2 (breakpoints between Bat2-Tnf segments of S/D interval). Bone marrow cells of B 10.SP2, 9347 and R40 mice were rejected by B6 H2b host NK cells as if B6 NK cells recognize codominantly inherited Dsp2 on donor BMC. 9347 × B6 F1 but not R40 X B6 BMC were accepted by B6 hosts, suggesting that Db plus a Bat2-Tnfb peptide from 9347 but not of R40 form a protective motif to prevent rejection by B6 host. Therefore 9347 and R40 mice were crossed to DBA.Db(H2dDb) transgenic mice to produce Kb/dDsp2/dLd, Db F1 mice; their BMC cells were rejected by B6 but were accepted by D8(H2bDd) hosts as if Dd was the target BMC from F2 donors that were H29347+(or even H2R40+), H2d-, Db+ grew in B6 hosts; BMC from Db- donors were rejected. Db plus peptides from non-H2d DBA genes may generate protective motifs for Dsp2 but not for Dd targets. Thus specific peptides and class I molecules are apparently required to create negative signaling motifs for NK cells to prevent rejection of BMC. BMC Donor 5-Day 125IUdR Uptake in Spleens Strain K D L Hosts: B6 D8: H2b, Dd R40 or 9347 b sp2 B6 × R40 b b/sp2 - NT B6 × 9347 b Wsp2 + NT 9347 × DBA.Db F1 b/d Sp2/d,b d - + 9347 × DBA.Db F2 b sp2, b+ + NT 9347 × DBA.Db F2 b sp2, b- - NT.",
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AU - Liu, J.

AU - Sentman, C.

AU - Fischer Lindahl, K.

AU - Kumar, V.

AU - Bennett, M.

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N2 - Crosses between wild type spretus and BIO H2b mice produced congenic B10.SP2 and intra-H2 recombinant 9347 and R40 mice. 9347 and R40 mice are KISb/DM3sp2 (breakpoints between Bat2-Tnf segments of S/D interval). Bone marrow cells of B 10.SP2, 9347 and R40 mice were rejected by B6 H2b host NK cells as if B6 NK cells recognize codominantly inherited Dsp2 on donor BMC. 9347 × B6 F1 but not R40 X B6 BMC were accepted by B6 hosts, suggesting that Db plus a Bat2-Tnfb peptide from 9347 but not of R40 form a protective motif to prevent rejection by B6 host. Therefore 9347 and R40 mice were crossed to DBA.Db(H2dDb) transgenic mice to produce Kb/dDsp2/dLd, Db F1 mice; their BMC cells were rejected by B6 but were accepted by D8(H2bDd) hosts as if Dd was the target BMC from F2 donors that were H29347+(or even H2R40+), H2d-, Db+ grew in B6 hosts; BMC from Db- donors were rejected. Db plus peptides from non-H2d DBA genes may generate protective motifs for Dsp2 but not for Dd targets. Thus specific peptides and class I molecules are apparently required to create negative signaling motifs for NK cells to prevent rejection of BMC. BMC Donor 5-Day 125IUdR Uptake in Spleens Strain K D L Hosts: B6 D8: H2b, Dd R40 or 9347 b sp2 B6 × R40 b b/sp2 - NT B6 × 9347 b Wsp2 + NT 9347 × DBA.Db F1 b/d Sp2/d,b d - + 9347 × DBA.Db F2 b sp2, b+ + NT 9347 × DBA.Db F2 b sp2, b- - NT.

AB - Crosses between wild type spretus and BIO H2b mice produced congenic B10.SP2 and intra-H2 recombinant 9347 and R40 mice. 9347 and R40 mice are KISb/DM3sp2 (breakpoints between Bat2-Tnf segments of S/D interval). Bone marrow cells of B 10.SP2, 9347 and R40 mice were rejected by B6 H2b host NK cells as if B6 NK cells recognize codominantly inherited Dsp2 on donor BMC. 9347 × B6 F1 but not R40 X B6 BMC were accepted by B6 hosts, suggesting that Db plus a Bat2-Tnfb peptide from 9347 but not of R40 form a protective motif to prevent rejection by B6 host. Therefore 9347 and R40 mice were crossed to DBA.Db(H2dDb) transgenic mice to produce Kb/dDsp2/dLd, Db F1 mice; their BMC cells were rejected by B6 but were accepted by D8(H2bDd) hosts as if Dd was the target BMC from F2 donors that were H29347+(or even H2R40+), H2d-, Db+ grew in B6 hosts; BMC from Db- donors were rejected. Db plus peptides from non-H2d DBA genes may generate protective motifs for Dsp2 but not for Dd targets. Thus specific peptides and class I molecules are apparently required to create negative signaling motifs for NK cells to prevent rejection of BMC. BMC Donor 5-Day 125IUdR Uptake in Spleens Strain K D L Hosts: B6 D8: H2b, Dd R40 or 9347 b sp2 B6 × R40 b b/sp2 - NT B6 × 9347 b Wsp2 + NT 9347 × DBA.Db F1 b/d Sp2/d,b d - + 9347 × DBA.Db F2 b sp2, b+ + NT 9347 × DBA.Db F2 b sp2, b- - NT.

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