Db plus peptide protective motifs prevent rejection of D8P2+ but not Dd+ bone marrow cells(BMC) by B6 host natural killer(NK) cells

J. Liu, C. Sentman, K. Fischer Lindahl, V. Kumar, M. Bennett

Research output: Contribution to journalArticle

Abstract

Crosses between wild type spretus and BIO H2b mice produced congenic B10.SP2 and intra-H2 recombinant 9347 and R40 mice. 9347 and R40 mice are KISb/DM3sp2 (breakpoints between Bat2-Tnf segments of S/D interval). Bone marrow cells of B 10.SP2, 9347 and R40 mice were rejected by B6 H2b host NK cells as if B6 NK cells recognize codominantly inherited Dsp2 on donor BMC. 9347 × B6 F1 but not R40 X B6 BMC were accepted by B6 hosts, suggesting that Db plus a Bat2-Tnfb peptide from 9347 but not of R40 form a protective motif to prevent rejection by B6 host. Therefore 9347 and R40 mice were crossed to DBA.Db(H2dDb) transgenic mice to produce Kb/dDsp2/dLd, Db F1 mice; their BMC cells were rejected by B6 but were accepted by D8(H2bDd) hosts as if Dd was the target BMC from F2 donors that were H29347+(or even H2R40+), H2d-, Db+ grew in B6 hosts; BMC from Db- donors were rejected. Db plus peptides from non-H2d DBA genes may generate protective motifs for Dsp2 but not for Dd targets. Thus specific peptides and class I molecules are apparently required to create negative signaling motifs for NK cells to prevent rejection of BMC. BMC Donor 5-Day 125IUdR Uptake in Spleens Strain K D L Hosts: B6 D8: H2b, Dd R40 or 9347 b sp2 B6 × R40 b b/sp2 - NT B6 × 9347 b Wsp2 + NT 9347 × DBA.Db F1 b/d Sp2/d,b d - + 9347 × DBA.Db F2 b sp2, b+ + NT 9347 × DBA.Db F2 b sp2, b- - NT.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
Publication statusPublished - 1996

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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