Dual-Color Single-Cell Imaging of the Suprachiasmatic Nucleus Reveals a Circadian Role in Network Synchrony

Yongli Shan, John H. Abel, Yan Li, Mariko Izumo, Kimberly H. Cox, Byeongha Jeong, Seung Hee Yoo, David P. Olson, Francis J. Doyle, Joseph S. Takahashi

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Shan et al. generate a Cre-inducible mouse reporter with green and red luciferases fused to PER2, enabling cell-specific circadian measurements with a dual-color imaging device. Using genetically directed bioluminescence from AVP or VIP neurons in the SCN circadian pacemaker, they unravel distinct functions of the circadian clock in these neuronal subtypes.

Original languageEnglish (US)
Pages (from-to)164-179.e7
JournalNeuron
Volume108
Issue number1
DOIs
StatePublished - Oct 14 2020

Keywords

  • Per2 gene
  • SCN
  • bioluminescence imaging
  • circadian rhythms
  • luciferase
  • neuronal coupling
  • neuronal network
  • suprachiasmatic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

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