Dual-Color Single-Cell Imaging of the Suprachiasmatic Nucleus Reveals a Circadian Role in Network Synchrony

Yongli Shan; John H. Abel; Yan Li; Mariko Izumo; Kimberly H. Cox; Byeongha Jeong; Seung Hee Yoo; David P. Olson; Francis J. Doyle; Joseph S. Takahashi

doi:10.1016/j.neuron.2020.07.012

Dual-Color Single-Cell Imaging of the Suprachiasmatic Nucleus Reveals a Circadian Role in Network Synchrony

Yongli Shan, John H. Abel, Yan Li, Mariko Izumo, Kimberly H. Cox, Byeongha Jeong, Seung Hee Yoo, David P. Olson, Francis J. Doyle, Joseph S. Takahashi

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Shan et al. generate a Cre-inducible mouse reporter with green and red luciferases fused to PER2, enabling cell-specific circadian measurements with a dual-color imaging device. Using genetically directed bioluminescence from AVP or VIP neurons in the SCN circadian pacemaker, they unravel distinct functions of the circadian clock in these neuronal subtypes.

Original language	English (US)
Pages (from-to)	164-179.e7
Journal	Neuron
Volume	108
Issue number	1
DOIs	https://doi.org/10.1016/j.neuron.2020.07.012
State	Published - Oct 14 2020

Keywords

Per2 gene
SCN
bioluminescence imaging
circadian rhythms
luciferase
neuronal coupling
neuronal network
suprachiasmatic nucleus

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neuron.2020.07.012

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title = "Dual-Color Single-Cell Imaging of the Suprachiasmatic Nucleus Reveals a Circadian Role in Network Synchrony",

abstract = "Shan et al. generate a Cre-inducible mouse reporter with green and red luciferases fused to PER2, enabling cell-specific circadian measurements with a dual-color imaging device. Using genetically directed bioluminescence from AVP or VIP neurons in the SCN circadian pacemaker, they unravel distinct functions of the circadian clock in these neuronal subtypes.",

keywords = "Per2 gene, SCN, bioluminescence imaging, circadian rhythms, luciferase, neuronal coupling, neuronal network, suprachiasmatic nucleus",

author = "Yongli Shan and Abel, {John H.} and Yan Li and Mariko Izumo and Cox, {Kimberly H.} and Byeongha Jeong and Yoo, {Seung Hee} and Olson, {David P.} and Doyle, {Francis J.} and Takahashi, {Joseph S.}",

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year = "2020",

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doi = "10.1016/j.neuron.2020.07.012",

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T1 - Dual-Color Single-Cell Imaging of the Suprachiasmatic Nucleus Reveals a Circadian Role in Network Synchrony

AU - Shan, Yongli

AU - Abel, John H.

AU - Li, Yan

AU - Izumo, Mariko

AU - Cox, Kimberly H.

AU - Jeong, Byeongha

AU - Yoo, Seung Hee

AU - Olson, David P.

AU - Doyle, Francis J.

AU - Takahashi, Joseph S.

PY - 2020/10/14

Y1 - 2020/10/14

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AB - Shan et al. generate a Cre-inducible mouse reporter with green and red luciferases fused to PER2, enabling cell-specific circadian measurements with a dual-color imaging device. Using genetically directed bioluminescence from AVP or VIP neurons in the SCN circadian pacemaker, they unravel distinct functions of the circadian clock in these neuronal subtypes.

KW - Per2 gene

KW - SCN

KW - bioluminescence imaging

KW - circadian rhythms

KW - luciferase

KW - neuronal coupling

KW - neuronal network

KW - suprachiasmatic nucleus

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Dual-Color Single-Cell Imaging of the Suprachiasmatic Nucleus Reveals a Circadian Role in Network Synchrony

Abstract

Keywords

ASJC Scopus subject areas

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