Dual detection approach to a more accurate measure of relative purity in high-throughput characterization of compound collections

Andrew Lemoff, Bing Yan

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The accurate determination of compound purity is crucial for characterizing library purity, monitoring the stability of storage compounds, and obtaining meaningful high-throughput screening results. However, current high-throughput techniques for the determination of compound purity are inadequate. We evaluated on-line chromatography detectors, including UVTWC, UV214, and ELSD detectors, in a series of studies of 233 compound mixtures prepared with known compositions. Results indicate that both UVTWC and UV 214 overestimate the minor component in a mixture whereas ELSD underestimates the minor component. An average of UVTWC and ELSD purities gives a more accurate measure of the relative purity for a wide range of compounds in various purity ranges. This technique was applied to 959 compounds from our compound collection to more accurately determine their relative purity.

Original languageEnglish (US)
Pages (from-to)746-751
Number of pages6
JournalJournal of Combinatorial Chemistry
Volume10
Issue number5
DOIs
Publication statusPublished - Sep 2008

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ASJC Scopus subject areas

  • Chemistry(all)

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