Dual mechanisms of regulation of Na/H exchanger NHE-3 by parathyroid hormone in rat kidney

Lingzhi Fan, Michael R. Wiederkehr, Roberto Collazo, Huamin Wang, Ladonna A. Crowder, Orson W. Moe

Research output: Contribution to journalArticle

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Abstract

Parathyroid hormone (PTH) is a potent inhibitor of mammalian renal proximal tubule sodium absorption via suppression of the apical membrane Na/H exchanger (NHE-3). We examined the mechanisms by which PTH inhibits NHE-3 activity by giving an acute intravenous PTH bolus to parathyroidectomized rats. Parathyroidectomy per se increased apical membrane NHE-3 activity and antigen. Acute infusion of PTH caused a time-dependent decrease in NHE-3 activity as early as 30 min. Decrease in NHE-3 activity at 30 and 60 min was accompanied by increased NHE-3 phosphorylation. In contrast to the rapid changes in NHE-3 activity and phosphorylation, decrease in apical membrane NHE-3 antigen was not detectable until 4-12 h after the PTH bolus. The decrease in apical membrane NHE-3 occurred in the absence of changes in total renal cortical NHE-3 antigen. Pretreatment of the animals with the microtubule-disrupting agent colchicine blocked the PTH-induced decrease in apical NHE-3 antigen. We propose that PTH acutely cause a decrease in NHE-3 intrinsic transport activity possibly via a phosphorylation-dependent mechanism followed by a decrease in apical membrane NHE-3 antigen via changes in protein trafficking.

Original languageEnglish (US)
Pages (from-to)11289-11295
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number16
DOIs
StatePublished - Apr 16 1999

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Sodium-Hydrogen Antiporter
Parathyroid Hormone
Rats
Kidney
Phosphorylation
Membranes
Antigens
Parathyroidectomy
Proximal Kidney Tubule
Colchicine
Protein Transport
Microtubules
Animals
Sodium

ASJC Scopus subject areas

  • Biochemistry

Cite this

Dual mechanisms of regulation of Na/H exchanger NHE-3 by parathyroid hormone in rat kidney. / Fan, Lingzhi; Wiederkehr, Michael R.; Collazo, Roberto; Wang, Huamin; Crowder, Ladonna A.; Moe, Orson W.

In: Journal of Biological Chemistry, Vol. 274, No. 16, 16.04.1999, p. 11289-11295.

Research output: Contribution to journalArticle

Fan, Lingzhi ; Wiederkehr, Michael R. ; Collazo, Roberto ; Wang, Huamin ; Crowder, Ladonna A. ; Moe, Orson W. / Dual mechanisms of regulation of Na/H exchanger NHE-3 by parathyroid hormone in rat kidney. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 16. pp. 11289-11295.
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