Duodenal ulcer-related antigens from Helicobacter pylori

Immunoproteome and protein microarray approaches

Yu Fen Lin, Chun Yi Chen, Mong Hsun Tsai, Ming Shiang Wu, Yu Chun Wang, Eric Y. Chuang, Jaw Town Lin, Pan Chyr Yang, Lu Ping Chow

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Helicobacter pylori is an important risk factor of duodenal ulcer (DU). Although many virulence factors of H. pylori have been identified, few have been reported to show an association with the pathogenesis of DU. The aims of this study were to identify H. pylori antigens showing a high seropositivity in DU and to develop a platform for rapid and easy diagnosis for DU. Because DU and gastric cancer (GC) are considered clinical divergent gastroduodenal diseases, we compared two-dimensional immunoblots of an acid-glycine extract of an H. pylori strain from a patient with DU probed with serum samples from 10 patients with DU and 10 with GC to identify DU-related antigens. Of the 11 proteins that were strongly recognized by serum IgG from DU patients, translation elongation factor EF-G (FusA), catalase (KatA), and urease α subunit (UreA) were identified as DU-related antigens, showing a higher seropositivity in DU samples (n = 124) than in GC samples (n = 95) (FusA, 70.2 versus 45.3%; KatA, 50.8 versus 41.1%; UreA, 44.4 versus 27.4%). In addition, we found that the use of multiple antigens improved the discrimination between patients with DU and those with GC as the odds ratios increased from 1.82 (95% confidence interval (CI), 0.79-4.21; p = 0.1607) for seropositivity for FusA, KatA, or UreA alone to 4.95 (95% Cl, 2.05-12.0; p = 0.0004) for two of the three antigens and to 5.71 (95% Cl, 1.86-17.6; p = 0.0024) for all three antigens. Moreover a protein array containing the three DU-related antigens was developed to test the idea of using multiple biomarkers in diagnosis. We conclude that FusA, KatA, and UreA are DU-related antigens of H. pylori, and the combination of these on a protein array provided a rapid and convenient method for detecting serum antibody patterns of DU patients.

Original languageEnglish (US)
Pages (from-to)1018-1026
Number of pages9
JournalMolecular and Cellular Proteomics
Volume6
Issue number6
DOIs
StatePublished - Jun 2007

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Protein Array Analysis
Microarrays
Duodenal Ulcer
Helicobacter pylori
Urease
Antigens
Proteins
Stomach Neoplasms
Peptide Elongation Factor G
Peptide Elongation Factors
Complement Factor H
Biomarkers
Virulence Factors
Catalase
Glycine
Immunoglobulin G
Association reactions
Duodenal Neoplasms
Serum
Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lin, Y. F., Chen, C. Y., Tsai, M. H., Wu, M. S., Wang, Y. C., Chuang, E. Y., ... Chow, L. P. (2007). Duodenal ulcer-related antigens from Helicobacter pylori: Immunoproteome and protein microarray approaches. Molecular and Cellular Proteomics, 6(6), 1018-1026. https://doi.org/10.1074/mcp.M700009-MCP200

Duodenal ulcer-related antigens from Helicobacter pylori : Immunoproteome and protein microarray approaches. / Lin, Yu Fen; Chen, Chun Yi; Tsai, Mong Hsun; Wu, Ming Shiang; Wang, Yu Chun; Chuang, Eric Y.; Lin, Jaw Town; Yang, Pan Chyr; Chow, Lu Ping.

In: Molecular and Cellular Proteomics, Vol. 6, No. 6, 06.2007, p. 1018-1026.

Research output: Contribution to journalArticle

Lin, YF, Chen, CY, Tsai, MH, Wu, MS, Wang, YC, Chuang, EY, Lin, JT, Yang, PC & Chow, LP 2007, 'Duodenal ulcer-related antigens from Helicobacter pylori: Immunoproteome and protein microarray approaches', Molecular and Cellular Proteomics, vol. 6, no. 6, pp. 1018-1026. https://doi.org/10.1074/mcp.M700009-MCP200
Lin, Yu Fen ; Chen, Chun Yi ; Tsai, Mong Hsun ; Wu, Ming Shiang ; Wang, Yu Chun ; Chuang, Eric Y. ; Lin, Jaw Town ; Yang, Pan Chyr ; Chow, Lu Ping. / Duodenal ulcer-related antigens from Helicobacter pylori : Immunoproteome and protein microarray approaches. In: Molecular and Cellular Proteomics. 2007 ; Vol. 6, No. 6. pp. 1018-1026.
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