Dynamic assembly and activation of estrogen receptor α enhancers through coregulator switching

Shino Murakami, Anusha Nagari, W. Lee Kraus

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Although many features of active transcriptional enhancers have been defined by genomic assays, we lack a clear understanding of the order of events leading to enhancer formation and activation as well as the dynamics of co-regulator interactions within the enhancer complex. Here, we used selective loss-or gain-of-function mutants of estrogen receptor α (ERα) to define two distinct phases of ligand-dependent enhancer formation. In the first phase (0-20 min), p300 is recruited to ERα by Mediator as well as p300’s acetylhistone-binding bromodomain to promote initial enhancer formation, which is not competent for sustained activation. In the second phase (20-45 min), p300 is recruited to ERα by steroid receptor coregulators (SRCs) for enhancer maturation and maintenance. Successful transition between these two phases (“coregulator switching”) is required for proper enhancer function. Failure to recruit p300 during either phase leads to abortive enhancer formation and a lack of target gene expression. Our results reveal an ordered and cooperative assembly of ERα enhancers requiring functional interplay among p300, Mediator, and SRCs, which has implications for hormone-dependent gene regulation in breast cancers. More broadly, our results demonstrate the unexpectedly dynamic nature of coregulator interactions within enhancer complexes, which are likely to be a defining feature of all enhancers.

Original languageEnglish (US)
Pages (from-to)1535-1548
Number of pages14
JournalGenes and Development
Volume31
Issue number15
DOIs
Publication statusPublished - Aug 1 2017

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Keywords

  • Breast cancer
  • Coregulator
  • Enhancer
  • Estrogen receptor α (ERα)
  • p300/CBP
  • Transcription

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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