Dynamic association of proteasomal machinery with the centrosome

Christian W. Wigley, Rosalind P. Fabunmi, Min Goo Lee, Christopher R. Marino, Shmuel Muallem, George N. DeMartino, Philip J. Thomas

Research output: Contribution to journalArticle

396 Citations (Scopus)

Abstract

Although the number of pathologies known to arise from the inappropriate folding of proteins continues to grow, mechanisms underlying the recognition and ultimate disposition of misfolded polypeptides remain obscure. For example, how and where such substrates are identified and processed is unknown. We report here the identification of a specific subcellular structure in which, under basal conditions, the 20S proteasome, the PA700 and PA28 (700- and 180-kD proteasome activator complexes, respectively), ubiquitin, Hsp70 and Hsp90 (70- and 90-kD heat shock protein, respectively) concentrate in HEK 293 and HeLa cells. The structure is perinuclear, surrounded by endoplasmic reticulum, adjacent to the Golgi, and colocalizes with γ-tubulin, an established centrosomal marker. Density gradient fractions containing purified centrosomes are enriched in proteasomal components and cell stress chaperones. The centrosome-associated structure enlarges in response to inhibition of proteasome activity and the level of misfolded proteins. For example, folding mutants of CFTR form large inclusions which arise from the centrosome upon inhibition of proteasome activity. At high levels of misfolded protein, the structure not only expands but also extensively recruits the cytosolic pools of ubiquitin, Hsp70, PA700, PA28, and the 20S proteasome. Thus, the centrosome may act as a scaffold, which concentrates and recruits the systems which act as censors and modulators of the balance between folding, aggregation, and degradation.

Original languageEnglish (US)
Pages (from-to)481-490
Number of pages10
JournalJournal of Cell Biology
Volume145
Issue number3
DOIs
StatePublished - May 3 1999

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Centrosome
Proteasome Endopeptidase Complex
Ubiquitin
HSP90 Heat-Shock Proteins
HEK293 Cells
Protein Folding
Cellular Structures
Tubulin
HeLa Cells
Endoplasmic Reticulum
Proteins
Pathology
Peptides

Keywords

  • Centrosome
  • CFTR
  • Inclusions
  • Proteasome
  • Protein misfolding

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dynamic association of proteasomal machinery with the centrosome. / Wigley, Christian W.; Fabunmi, Rosalind P.; Lee, Min Goo; Marino, Christopher R.; Muallem, Shmuel; DeMartino, George N.; Thomas, Philip J.

In: Journal of Cell Biology, Vol. 145, No. 3, 03.05.1999, p. 481-490.

Research output: Contribution to journalArticle

Wigley, Christian W. ; Fabunmi, Rosalind P. ; Lee, Min Goo ; Marino, Christopher R. ; Muallem, Shmuel ; DeMartino, George N. ; Thomas, Philip J. / Dynamic association of proteasomal machinery with the centrosome. In: Journal of Cell Biology. 1999 ; Vol. 145, No. 3. pp. 481-490.
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