Dynamic gene expression analysis links melanocyte growth arrest with nevogenesis

Guang Yang, Khanh Thieu, Kenneth Y. Tsai, Adriano Piris, Durga Udayakumar, Ching Ni Jenny Njauw, Marco F. Ramoni, Hensin Tsao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Like all primary cells in vitro, normal human melanocytes exhibit a physiologic decay in proliferative potential as it transitions to a growth-arrested state. The underlying transcriptional program(s) that regulate this phenotypic change is largely unknown. To identify molecular determinants of this process, we performed a Bayesian-based dynamic gene expression analysis on primary melanocytes undergoing proliferative arrest. This analysis revealed several related clusters whose expression behavior correlated with the melanocyte growth kinetics; we designated these clusters the melanocyte growth arrest program (MGAP). These MGAP genes were preferentially represented in benign melanocytic nevi over melanomas and selectively mapped to the hepatocyte fibrosis pathway. This transcriptional relationship between melanocyte growth stasis, nevus biology, and fibrogenic signaling was further validated in vivo by the demonstration of strong pericellular collagen deposition within benign nevi but not melanomas. Taken together, our study provides a novel view of fibroplasia in both melanocyte biology and nevogenesis.

Original languageEnglish (US)
Pages (from-to)9029-9037
Number of pages9
JournalCancer Research
Volume69
Issue number23
DOIs
StatePublished - Dec 1 2009

Fingerprint

Melanocytes
Gene Expression
Growth
Nevi and Melanomas
Pigmented Nevus
Nevus
Hepatocytes
Fibrosis
Collagen
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Yang, G., Thieu, K., Tsai, K. Y., Piris, A., Udayakumar, D., Njauw, C. N. J., ... Tsao, H. (2009). Dynamic gene expression analysis links melanocyte growth arrest with nevogenesis. Cancer Research, 69(23), 9029-9037. https://doi.org/10.1158/0008-5472.CAN-09-0783

Dynamic gene expression analysis links melanocyte growth arrest with nevogenesis. / Yang, Guang; Thieu, Khanh; Tsai, Kenneth Y.; Piris, Adriano; Udayakumar, Durga; Njauw, Ching Ni Jenny; Ramoni, Marco F.; Tsao, Hensin.

In: Cancer Research, Vol. 69, No. 23, 01.12.2009, p. 9029-9037.

Research output: Contribution to journalArticle

Yang, G, Thieu, K, Tsai, KY, Piris, A, Udayakumar, D, Njauw, CNJ, Ramoni, MF & Tsao, H 2009, 'Dynamic gene expression analysis links melanocyte growth arrest with nevogenesis', Cancer Research, vol. 69, no. 23, pp. 9029-9037. https://doi.org/10.1158/0008-5472.CAN-09-0783
Yang, Guang ; Thieu, Khanh ; Tsai, Kenneth Y. ; Piris, Adriano ; Udayakumar, Durga ; Njauw, Ching Ni Jenny ; Ramoni, Marco F. ; Tsao, Hensin. / Dynamic gene expression analysis links melanocyte growth arrest with nevogenesis. In: Cancer Research. 2009 ; Vol. 69, No. 23. pp. 9029-9037.
@article{2846bb0ec45f4383a45caf59b1510f9e,
title = "Dynamic gene expression analysis links melanocyte growth arrest with nevogenesis",
abstract = "Like all primary cells in vitro, normal human melanocytes exhibit a physiologic decay in proliferative potential as it transitions to a growth-arrested state. The underlying transcriptional program(s) that regulate this phenotypic change is largely unknown. To identify molecular determinants of this process, we performed a Bayesian-based dynamic gene expression analysis on primary melanocytes undergoing proliferative arrest. This analysis revealed several related clusters whose expression behavior correlated with the melanocyte growth kinetics; we designated these clusters the melanocyte growth arrest program (MGAP). These MGAP genes were preferentially represented in benign melanocytic nevi over melanomas and selectively mapped to the hepatocyte fibrosis pathway. This transcriptional relationship between melanocyte growth stasis, nevus biology, and fibrogenic signaling was further validated in vivo by the demonstration of strong pericellular collagen deposition within benign nevi but not melanomas. Taken together, our study provides a novel view of fibroplasia in both melanocyte biology and nevogenesis.",
author = "Guang Yang and Khanh Thieu and Tsai, {Kenneth Y.} and Adriano Piris and Durga Udayakumar and Njauw, {Ching Ni Jenny} and Ramoni, {Marco F.} and Hensin Tsao",
year = "2009",
month = "12",
day = "1",
doi = "10.1158/0008-5472.CAN-09-0783",
language = "English (US)",
volume = "69",
pages = "9029--9037",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "23",

}

TY - JOUR

T1 - Dynamic gene expression analysis links melanocyte growth arrest with nevogenesis

AU - Yang, Guang

AU - Thieu, Khanh

AU - Tsai, Kenneth Y.

AU - Piris, Adriano

AU - Udayakumar, Durga

AU - Njauw, Ching Ni Jenny

AU - Ramoni, Marco F.

AU - Tsao, Hensin

PY - 2009/12/1

Y1 - 2009/12/1

N2 - Like all primary cells in vitro, normal human melanocytes exhibit a physiologic decay in proliferative potential as it transitions to a growth-arrested state. The underlying transcriptional program(s) that regulate this phenotypic change is largely unknown. To identify molecular determinants of this process, we performed a Bayesian-based dynamic gene expression analysis on primary melanocytes undergoing proliferative arrest. This analysis revealed several related clusters whose expression behavior correlated with the melanocyte growth kinetics; we designated these clusters the melanocyte growth arrest program (MGAP). These MGAP genes were preferentially represented in benign melanocytic nevi over melanomas and selectively mapped to the hepatocyte fibrosis pathway. This transcriptional relationship between melanocyte growth stasis, nevus biology, and fibrogenic signaling was further validated in vivo by the demonstration of strong pericellular collagen deposition within benign nevi but not melanomas. Taken together, our study provides a novel view of fibroplasia in both melanocyte biology and nevogenesis.

AB - Like all primary cells in vitro, normal human melanocytes exhibit a physiologic decay in proliferative potential as it transitions to a growth-arrested state. The underlying transcriptional program(s) that regulate this phenotypic change is largely unknown. To identify molecular determinants of this process, we performed a Bayesian-based dynamic gene expression analysis on primary melanocytes undergoing proliferative arrest. This analysis revealed several related clusters whose expression behavior correlated with the melanocyte growth kinetics; we designated these clusters the melanocyte growth arrest program (MGAP). These MGAP genes were preferentially represented in benign melanocytic nevi over melanomas and selectively mapped to the hepatocyte fibrosis pathway. This transcriptional relationship between melanocyte growth stasis, nevus biology, and fibrogenic signaling was further validated in vivo by the demonstration of strong pericellular collagen deposition within benign nevi but not melanomas. Taken together, our study provides a novel view of fibroplasia in both melanocyte biology and nevogenesis.

UR - http://www.scopus.com/inward/record.url?scp=71549129521&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71549129521&partnerID=8YFLogxK

U2 - 10.1158/0008-5472.CAN-09-0783

DO - 10.1158/0008-5472.CAN-09-0783

M3 - Article

C2 - 19903842

AN - SCOPUS:71549129521

VL - 69

SP - 9029

EP - 9037

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 23

ER -