Dynamic recognition of the mRNA cap by Saccharomyces cerevisiae eIF4E

Seán E. O'Leary, Alexey Petrov, Jin Chen, Joseph D. Puglisi

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Recognition of the mRNA 5′ m7G(5′)ppp(5′)N cap is key to translation initiation for most eukaryotic mRNAs. The cap is bound by the eIF4F complex, consisting of a cap-binding protein (eIF4E), a "scaffold" protein (eIF4G), and an RNA helicase (eIF4A). As a central early step in initiation, regulation of eIF4F is crucial for cellular viability. Although the structure and function of eIF4E have been defined, a dynamic mechanistic picture of its activity at the molecular level in the eIF4F×mRNA complex is still unavailable. Here, using single-molecule fluorescence, we measured the effects of Saccharomyces cerevisiae eIF4F factors, mRNA secondary structure, and the poly(A)-binding protein Pab1p on eIF4E-mRNA binding dynamics. Our data provide an integrated picture of how eIF4G and mRNA structure modulate eIF4E-mRNA interaction, and uncover an eIF4G- and poly(A)-independent activity of poly(A)-binding protein that prolongs the eIF4E×mRNA complex lifetime.

Original languageEnglish (US)
Pages (from-to)2197-2207
Number of pages11
JournalStructure
Volume21
Issue number12
DOIs
StatePublished - Dec 3 2013
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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