Dynamic recognition of the mRNA cap by Saccharomyces cerevisiae eIF4E

Seán E. O'Leary, Alexey Petrov, Jin Chen, Joseph D. Puglisi

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


Recognition of the mRNA 5′ m7G(5′)ppp(5′)N cap is key to translation initiation for most eukaryotic mRNAs. The cap is bound by the eIF4F complex, consisting of a cap-binding protein (eIF4E), a "scaffold" protein (eIF4G), and an RNA helicase (eIF4A). As a central early step in initiation, regulation of eIF4F is crucial for cellular viability. Although the structure and function of eIF4E have been defined, a dynamic mechanistic picture of its activity at the molecular level in the eIF4F×mRNA complex is still unavailable. Here, using single-molecule fluorescence, we measured the effects of Saccharomyces cerevisiae eIF4F factors, mRNA secondary structure, and the poly(A)-binding protein Pab1p on eIF4E-mRNA binding dynamics. Our data provide an integrated picture of how eIF4G and mRNA structure modulate eIF4E-mRNA interaction, and uncover an eIF4G- and poly(A)-independent activity of poly(A)-binding protein that prolongs the eIF4E×mRNA complex lifetime.

Original languageEnglish (US)
Pages (from-to)2197-2207
Number of pages11
Issue number12
StatePublished - Dec 3 2013
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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