Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity

Tatsuya Ingi, Andrejs M. Krumins, Peter Chidiac, Greg M. Brothers, Stephen Chung, Bryan E. Snow, Carol A. Barnes, Anthony A. Lanahan, David P. Siderovski, Elliott M. Ross, Alfred G. Gilman, Paul F. Worley

Research output: Contribution to journalArticle

247 Citations (Scopus)

Abstract

Long-term neuronal plasticity is known to be dependent on rapid de novo synthesis of mRNA and protein, and recent studies provide insight into the molecules involved in this response. Here, we demonstrate that mRNA encoding a member of the regulator of G-protein signaling (RGS) family, RGS2, is rapidly induced in neurons of the hippocampus, cortex, and striatum in response to stimuli that evoke plasticity. Although several members of the RGS family are expressed in brain with discrete neuronal localizations, RGS2 appears unique in that its expression is dynamically responsive to neuronal activity. In biochemical assays, RGS2 stimulates the GTPase activity of the a subunit of G(q) and G(i)1. The effect on G(i)1 was observed only after reconstitution of the protein in phospholipid vesicles containing M2 muscarinic acetylcholine receptors. RGS2 also inhibits both G(q)- and G(i)- dependent responses in transfected cells. These studies suggest a novel mechanism linking neuronal activity and signal transduction.

Original languageEnglish (US)
Pages (from-to)7178-7188
Number of pages11
JournalJournal of Neuroscience
Volume18
Issue number18
StatePublished - Sep 15 1998

Fingerprint

GTP-Binding Protein Regulators
Neuronal Plasticity
GTP-Binding Proteins
Messenger RNA
GTP Phosphohydrolases
Muscarinic Receptors
Signal Transduction
Hippocampus
Phospholipids
Proteins
Neurons
Brain

Keywords

  • G-protein
  • GTPase- activating proteins
  • Immediate early genes
  • MAP kinase
  • Neuronal plasticity
  • RGS
  • Seizure

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ingi, T., Krumins, A. M., Chidiac, P., Brothers, G. M., Chung, S., Snow, B. E., ... Worley, P. F. (1998). Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity. Journal of Neuroscience, 18(18), 7178-7188.

Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity. / Ingi, Tatsuya; Krumins, Andrejs M.; Chidiac, Peter; Brothers, Greg M.; Chung, Stephen; Snow, Bryan E.; Barnes, Carol A.; Lanahan, Anthony A.; Siderovski, David P.; Ross, Elliott M.; Gilman, Alfred G.; Worley, Paul F.

In: Journal of Neuroscience, Vol. 18, No. 18, 15.09.1998, p. 7178-7188.

Research output: Contribution to journalArticle

Ingi, T, Krumins, AM, Chidiac, P, Brothers, GM, Chung, S, Snow, BE, Barnes, CA, Lanahan, AA, Siderovski, DP, Ross, EM, Gilman, AG & Worley, PF 1998, 'Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity', Journal of Neuroscience, vol. 18, no. 18, pp. 7178-7188.
Ingi T, Krumins AM, Chidiac P, Brothers GM, Chung S, Snow BE et al. Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity. Journal of Neuroscience. 1998 Sep 15;18(18):7178-7188.
Ingi, Tatsuya ; Krumins, Andrejs M. ; Chidiac, Peter ; Brothers, Greg M. ; Chung, Stephen ; Snow, Bryan E. ; Barnes, Carol A. ; Lanahan, Anthony A. ; Siderovski, David P. ; Ross, Elliott M. ; Gilman, Alfred G. ; Worley, Paul F. / Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity. In: Journal of Neuroscience. 1998 ; Vol. 18, No. 18. pp. 7178-7188.
@article{20a1004e61b743ddbcfd87ce442ea4c5,
title = "Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity",
abstract = "Long-term neuronal plasticity is known to be dependent on rapid de novo synthesis of mRNA and protein, and recent studies provide insight into the molecules involved in this response. Here, we demonstrate that mRNA encoding a member of the regulator of G-protein signaling (RGS) family, RGS2, is rapidly induced in neurons of the hippocampus, cortex, and striatum in response to stimuli that evoke plasticity. Although several members of the RGS family are expressed in brain with discrete neuronal localizations, RGS2 appears unique in that its expression is dynamically responsive to neuronal activity. In biochemical assays, RGS2 stimulates the GTPase activity of the a subunit of G(q) and G(i)1. The effect on G(i)1 was observed only after reconstitution of the protein in phospholipid vesicles containing M2 muscarinic acetylcholine receptors. RGS2 also inhibits both G(q)- and G(i)- dependent responses in transfected cells. These studies suggest a novel mechanism linking neuronal activity and signal transduction.",
keywords = "G-protein, GTPase- activating proteins, Immediate early genes, MAP kinase, Neuronal plasticity, RGS, Seizure",
author = "Tatsuya Ingi and Krumins, {Andrejs M.} and Peter Chidiac and Brothers, {Greg M.} and Stephen Chung and Snow, {Bryan E.} and Barnes, {Carol A.} and Lanahan, {Anthony A.} and Siderovski, {David P.} and Ross, {Elliott M.} and Gilman, {Alfred G.} and Worley, {Paul F.}",
year = "1998",
month = "9",
day = "15",
language = "English (US)",
volume = "18",
pages = "7178--7188",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "18",

}

TY - JOUR

T1 - Dynamic regulation of RGS2 suggests a novel mechanism in G-protein signaling and neuronal plasticity

AU - Ingi, Tatsuya

AU - Krumins, Andrejs M.

AU - Chidiac, Peter

AU - Brothers, Greg M.

AU - Chung, Stephen

AU - Snow, Bryan E.

AU - Barnes, Carol A.

AU - Lanahan, Anthony A.

AU - Siderovski, David P.

AU - Ross, Elliott M.

AU - Gilman, Alfred G.

AU - Worley, Paul F.

PY - 1998/9/15

Y1 - 1998/9/15

N2 - Long-term neuronal plasticity is known to be dependent on rapid de novo synthesis of mRNA and protein, and recent studies provide insight into the molecules involved in this response. Here, we demonstrate that mRNA encoding a member of the regulator of G-protein signaling (RGS) family, RGS2, is rapidly induced in neurons of the hippocampus, cortex, and striatum in response to stimuli that evoke plasticity. Although several members of the RGS family are expressed in brain with discrete neuronal localizations, RGS2 appears unique in that its expression is dynamically responsive to neuronal activity. In biochemical assays, RGS2 stimulates the GTPase activity of the a subunit of G(q) and G(i)1. The effect on G(i)1 was observed only after reconstitution of the protein in phospholipid vesicles containing M2 muscarinic acetylcholine receptors. RGS2 also inhibits both G(q)- and G(i)- dependent responses in transfected cells. These studies suggest a novel mechanism linking neuronal activity and signal transduction.

AB - Long-term neuronal plasticity is known to be dependent on rapid de novo synthesis of mRNA and protein, and recent studies provide insight into the molecules involved in this response. Here, we demonstrate that mRNA encoding a member of the regulator of G-protein signaling (RGS) family, RGS2, is rapidly induced in neurons of the hippocampus, cortex, and striatum in response to stimuli that evoke plasticity. Although several members of the RGS family are expressed in brain with discrete neuronal localizations, RGS2 appears unique in that its expression is dynamically responsive to neuronal activity. In biochemical assays, RGS2 stimulates the GTPase activity of the a subunit of G(q) and G(i)1. The effect on G(i)1 was observed only after reconstitution of the protein in phospholipid vesicles containing M2 muscarinic acetylcholine receptors. RGS2 also inhibits both G(q)- and G(i)- dependent responses in transfected cells. These studies suggest a novel mechanism linking neuronal activity and signal transduction.

KW - G-protein

KW - GTPase- activating proteins

KW - Immediate early genes

KW - MAP kinase

KW - Neuronal plasticity

KW - RGS

KW - Seizure

UR - http://www.scopus.com/inward/record.url?scp=17344374133&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17344374133&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 7178

EP - 7188

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 18

ER -