Dynamic viral dissemination in mice infected with yellow fever virus strain 17D

Andrea K. Erickson, Julie K. Pfeiffer

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Arboviruses such as yellow fever virus (YFV) are transmitted between arthropod vectors and vertebrate hosts. While barriers limiting arbovirus population diversity have been observed in mosquitoes, whether barriers exist in vertebrate hosts is unclear. To investigate whether arboviruses encounter bottlenecks during dissemination in the vertebrate host, we infected immunocompetent mice and immune-deficient mice lacking alpha/beta interferon (IFN-α/β) receptors (IFNAR-/- mice) with a pool of genetically marked viruses to evaluate dissemination and host barriers. We used the live attenuated vaccine strain YFV-17D, which contains many mutations compared with virulent YFV. We found that intramuscularly injected immunocompetent mice did not develop disease and that viral dissemination was restricted. Conversely, 32% of intramuscularly injected IFNAR-/- mice developed disease. By following the genetically marked viruses over time, we found broad dissemination in IFNAR-/- mice followed by clearance. The patterns of viral dissemination were similar in mice that developed disease and mice that did not develop disease. Unlike our previous results with poliovirus, these results suggest that YFV-17D encounters no major barriers during dissemination within a vertebrate host in the absence of the type I IFN response.

Original languageEnglish (US)
Pages (from-to)12392-12397
Number of pages6
JournalJournal of Virology
Volume87
Issue number22
DOIs
StatePublished - 2013

Fingerprint

Yellow fever virus
mice
Arboviruses
arboviruses
Vertebrates
vertebrates
live vaccines
Arthropod Vectors
Interferon alpha-beta Receptor
interferon-beta
Viruses
Enterovirus C
viruses
Attenuated Vaccines
Poliovirus
Virus Diseases
Culicidae
arthropods
mutation
Mutation

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Medicine(all)

Cite this

Dynamic viral dissemination in mice infected with yellow fever virus strain 17D. / Erickson, Andrea K.; Pfeiffer, Julie K.

In: Journal of Virology, Vol. 87, No. 22, 2013, p. 12392-12397.

Research output: Contribution to journalArticle

@article{f6bd1939d8fd46278cf5b59dfa96f16e,
title = "Dynamic viral dissemination in mice infected with yellow fever virus strain 17D",
abstract = "Arboviruses such as yellow fever virus (YFV) are transmitted between arthropod vectors and vertebrate hosts. While barriers limiting arbovirus population diversity have been observed in mosquitoes, whether barriers exist in vertebrate hosts is unclear. To investigate whether arboviruses encounter bottlenecks during dissemination in the vertebrate host, we infected immunocompetent mice and immune-deficient mice lacking alpha/beta interferon (IFN-α/β) receptors (IFNAR-/- mice) with a pool of genetically marked viruses to evaluate dissemination and host barriers. We used the live attenuated vaccine strain YFV-17D, which contains many mutations compared with virulent YFV. We found that intramuscularly injected immunocompetent mice did not develop disease and that viral dissemination was restricted. Conversely, 32{\%} of intramuscularly injected IFNAR-/- mice developed disease. By following the genetically marked viruses over time, we found broad dissemination in IFNAR-/- mice followed by clearance. The patterns of viral dissemination were similar in mice that developed disease and mice that did not develop disease. Unlike our previous results with poliovirus, these results suggest that YFV-17D encounters no major barriers during dissemination within a vertebrate host in the absence of the type I IFN response.",
author = "Erickson, {Andrea K.} and Pfeiffer, {Julie K.}",
year = "2013",
doi = "10.1128/JVI.02149-13",
language = "English (US)",
volume = "87",
pages = "12392--12397",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "22",

}

TY - JOUR

T1 - Dynamic viral dissemination in mice infected with yellow fever virus strain 17D

AU - Erickson, Andrea K.

AU - Pfeiffer, Julie K.

PY - 2013

Y1 - 2013

N2 - Arboviruses such as yellow fever virus (YFV) are transmitted between arthropod vectors and vertebrate hosts. While barriers limiting arbovirus population diversity have been observed in mosquitoes, whether barriers exist in vertebrate hosts is unclear. To investigate whether arboviruses encounter bottlenecks during dissemination in the vertebrate host, we infected immunocompetent mice and immune-deficient mice lacking alpha/beta interferon (IFN-α/β) receptors (IFNAR-/- mice) with a pool of genetically marked viruses to evaluate dissemination and host barriers. We used the live attenuated vaccine strain YFV-17D, which contains many mutations compared with virulent YFV. We found that intramuscularly injected immunocompetent mice did not develop disease and that viral dissemination was restricted. Conversely, 32% of intramuscularly injected IFNAR-/- mice developed disease. By following the genetically marked viruses over time, we found broad dissemination in IFNAR-/- mice followed by clearance. The patterns of viral dissemination were similar in mice that developed disease and mice that did not develop disease. Unlike our previous results with poliovirus, these results suggest that YFV-17D encounters no major barriers during dissemination within a vertebrate host in the absence of the type I IFN response.

AB - Arboviruses such as yellow fever virus (YFV) are transmitted between arthropod vectors and vertebrate hosts. While barriers limiting arbovirus population diversity have been observed in mosquitoes, whether barriers exist in vertebrate hosts is unclear. To investigate whether arboviruses encounter bottlenecks during dissemination in the vertebrate host, we infected immunocompetent mice and immune-deficient mice lacking alpha/beta interferon (IFN-α/β) receptors (IFNAR-/- mice) with a pool of genetically marked viruses to evaluate dissemination and host barriers. We used the live attenuated vaccine strain YFV-17D, which contains many mutations compared with virulent YFV. We found that intramuscularly injected immunocompetent mice did not develop disease and that viral dissemination was restricted. Conversely, 32% of intramuscularly injected IFNAR-/- mice developed disease. By following the genetically marked viruses over time, we found broad dissemination in IFNAR-/- mice followed by clearance. The patterns of viral dissemination were similar in mice that developed disease and mice that did not develop disease. Unlike our previous results with poliovirus, these results suggest that YFV-17D encounters no major barriers during dissemination within a vertebrate host in the absence of the type I IFN response.

UR - http://www.scopus.com/inward/record.url?scp=84886261937&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886261937&partnerID=8YFLogxK

U2 - 10.1128/JVI.02149-13

DO - 10.1128/JVI.02149-13

M3 - Article

VL - 87

SP - 12392

EP - 12397

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 22

ER -