Dynamics of atrial natriuretic factor-guanylate cyclase receptors and receptor-ligand complexes in cultured glomerular mesangial and renomedullary interstitial cells

Gou Y. Koh, Daniel R. Nussenzveig, Juraj Okolicany, Deborah A. Price, Thomas Maack

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Abstract

The dynamics of the guanylate cyclase receptor of atrial natriuretic factor (GCA-ANF receptor) were investigated in cultured glomerular mesangial and renomedullary interstitial cells from the rat. In these cells, the GCA-ANF receptor did not mediate internalization and lysosomal hydrolysis of 125I-ANF1-28 and did not undergo ligand-induced endocytosis. Glomerular mesangial cells were able, however, to mediate internalization and lysosomal hydrolysis of 125I-ANF1-28 via clearance ANF (C-ANF) receptors and to promote rapid receptor-mediated internalization and lysosomal hydrolysis of 125I-(Sar1) angiotensin II. Radioligand specifically bound to surface GCA-ANF receptors was rapidly dissociated at 37 °C (koff > 0.8 min-1), with a Q10(30-37 °C) > 6. The dissociation was markedly slower at subphysiological temperatures (Q10(4-30 °C), 2-3) or in the presence of 0.5 mM amiloride. The results demonstrate that the GCA-ANF receptor, contrary to C-ANF receptors and most other polypeptide hormone receptors, is a membrane resident protein that does not mediate internalization and lysosomal hydrolysis of ligand. The termination of the interaction of ANF with GCA-ANF receptors results from a physiological process that leads to rapid dissociation of receptor-ligand complexes. The unique dynamics of GCA-ANF receptor-ligand complexes are likely to contribute importantly to stimulus-response homeostasis ofANF.

Original languageEnglish (US)
Pages (from-to)11987-11994
Number of pages8
JournalJournal of Biological Chemistry
Volume267
Issue number17
StatePublished - Jun 15 1992

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Atrial Natriuretic Factor Receptors
Guanylate Cyclase
Atrial Natriuretic Factor
Ligands
Hydrolysis
Physiological Phenomena
Mesangial Cells
Peptide Hormones
Amiloride
Endocytosis
Angiotensin II
Rats
Membrane Proteins
Homeostasis
Membranes

ASJC Scopus subject areas

  • Biochemistry

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Dynamics of atrial natriuretic factor-guanylate cyclase receptors and receptor-ligand complexes in cultured glomerular mesangial and renomedullary interstitial cells. / Koh, Gou Y.; Nussenzveig, Daniel R.; Okolicany, Juraj; Price, Deborah A.; Maack, Thomas.

In: Journal of Biological Chemistry, Vol. 267, No. 17, 15.06.1992, p. 11987-11994.

Research output: Contribution to journalArticle

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