Dynamin self-assembly stimulates its GTPase activity

Dale E. Warnock, Jenny E. Hinshaw, Sandra L. Schmid

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

GTP hydrolysis by dynamin is required to drive coated vesicle budding at the plasma membrane. A diverse set of molecules including microtubules, grb2, and acidic phospholipids stimulate dynamin GTPase activity in vitro, although the physiological relevance of these effectors remains to be determined. Dynamin has been shown to assemble around microtubules, the most potent stimulatory molecule, into structures indistinguishable by electron microscopy from collars captured in vivo at the necks of endocytic coated pits. Under low ionic strength conditions purified dynamin self-assembles into rings and helical stacks of rings. Here we show that dynamin self- assembly stimulates its GTPase activity as much as 10-fold. Thus, we identify dynamin, itself, as the first effector of dynamin GTPase activity known to be physiologically relevant. Assembled dynamin's stimulated GTPase activity is not dependent on the direct interaction of high affinity GTP binding sites since a mutant defective in GTP binding and hydrolysis can coassemble with and stimulate GTP hydrolysis by wild-type dynamin. Finally, we find that GTP destabilizes assembled dynamin structures, suggesting that the activated rates of GTP hydrolysis reflect a continuing cycle of assembly, GTP hydrolysis, and disassembly.

Original languageEnglish (US)
Pages (from-to)22310-22314
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number37
DOIs
StatePublished - 1996

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Dynamins
GTP Phosphohydrolases
Self assembly
Guanosine Triphosphate
Hydrolysis
Microtubules
Coated Vesicles
Molecules
Cell membranes
Ionic strength
Osmolar Concentration
Electron microscopy
Phospholipids
Electron Microscopy
Neck
Binding Sites
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry

Cite this

Dynamin self-assembly stimulates its GTPase activity. / Warnock, Dale E.; Hinshaw, Jenny E.; Schmid, Sandra L.

In: Journal of Biological Chemistry, Vol. 271, No. 37, 1996, p. 22310-22314.

Research output: Contribution to journalArticle

Warnock, Dale E. ; Hinshaw, Jenny E. ; Schmid, Sandra L. / Dynamin self-assembly stimulates its GTPase activity. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 37. pp. 22310-22314.
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