Dysregulation of synaptic plasticity precedes appearance of morphological defects in a Pten conditional knockout mouse model of autism

Koichi Takeuchi, Michael J. Gertner, Jing Zhou, Luis F. Parada, Michael V L Bennett, R. Suzanne Zukin

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Abstract

The phosphoinositide signaling system is a crucial regulator of neural development, cell survival, and plasticity. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) negatively regulates phosphatidylinositol 3-kinase signaling and downstream targets. Nse-Cre Pten conditional knockout mice, in which Pten is ablated in granule cells of the dentate gyrus and pyramidal neurons of the hippocampal CA3, but not CA1, recapitulate many of the symptoms of humans with inactivating PTEN mutations, including progressive hypertrophy of the dentate gyrus and deficits in hippocampus- based social and cognitive behaviors. However, the impact of Pten loss on activity-dependent synaptic plasticity in this clinically relevant mouse model of Pten inactivation remains unclear. Here, we show that two phosphatidylinositol 3-kinase- and protein synthesis-dependent forms of synaptic plasticity, theta burstinduced long-term potentiation and metabotropic glutamate receptor (mGluR)-dependent long-term depression, are dysregulated at medial perforant path-to-dentate gyrus synapses of young Nse- Cre Pten conditional knockout mice before the onset of visible morphological abnormalities. In contrast, long-term potentiation and mGluR-dependent long-term depression are normal at CA3-CA1 pyramidal cell synapses at this age. Our results reveal that deletion of Pten in dentate granule cells dysregulates synaptic plasticity, a defect that may underlie abnormal social and cognitive behaviors observed in humans with Pten inactivating mutations and potentially other autism spectrum disorders.

Original languageEnglish (US)
Pages (from-to)4738-4743
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number12
DOIs
StatePublished - Mar 19 2013

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Neuronal Plasticity
Dentate Gyrus
Autistic Disorder
Knockout Mice
Phosphatidylinositol 3-Kinase
Metabotropic Glutamate Receptors
Long-Term Potentiation
Pyramidal Cells
Synapses
Depression
Perforant Pathway
Chromosomes, Human, Pair 10
Mutation
Phosphatidylinositols
Phosphoric Monoester Hydrolases
Hypertrophy
Hippocampus
Cell Survival
Proteins

ASJC Scopus subject areas

  • General

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Dysregulation of synaptic plasticity precedes appearance of morphological defects in a Pten conditional knockout mouse model of autism. / Takeuchi, Koichi; Gertner, Michael J.; Zhou, Jing; Parada, Luis F.; Bennett, Michael V L; Zukin, R. Suzanne.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 12, 19.03.2013, p. 4738-4743.

Research output: Contribution to journalArticle

Takeuchi, Koichi ; Gertner, Michael J. ; Zhou, Jing ; Parada, Luis F. ; Bennett, Michael V L ; Zukin, R. Suzanne. / Dysregulation of synaptic plasticity precedes appearance of morphological defects in a Pten conditional knockout mouse model of autism. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 12. pp. 4738-4743.
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