E6 and E7 expression from the HPV 18 LCR: Development of genital hyperplasia and neoplasia in transgenic mice

Sarah A Comerford, S. D. Maika, L. A. Laimins, A. Messing, H. P. Elsasser, Robert E Hammer

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Human papillomavirus type 18 infection is highly associated with malignant tumors of the genital tract. To investigate the tissue specificity of the HPV long control region (LCR) and the transforming ability of the E6-E7 oncoproteins, an HPV-18 transgene containing the viral LCR and E6 and E7 genes was introduced into mice. Three founder males exhibited enlarged seminal vesicles and preputial glands by 50 weeks of age. A line of transgenic mice was established by in vitro fertilization, and subsequent generations of transgenic males and females were monitored for lesions. Approximately 80% of hemizygous transgenic males exhibited enlarged seminal vesicles and preputial glands as early as 12 weeks of age. Histological examination indicated that this enlargement was due to distension by fluid, along with polyploid hyperplasia of the lining secretory epithelium. E6 and E7 transcripts were limited to affected organs and kidney. Approximately 41% of transgenic females developed cervical neoplasms between 1-2 years of age, Histologically, tumors were mesenchymal rather than epithelial in origin. E6 and E7 transcripts were restricted to cervical tumor tissue and kidney. These findings suggest that the HPV-18 LCR has an element(s) which directs expression specifically to the urogenital tract in transgenic mice.

Original languageEnglish (US)
Pages (from-to)587-597
Number of pages11
JournalOncogene
Volume10
Issue number3
StatePublished - 1995

Fingerprint

Human papillomavirus 18
Transgenic Mice
Hyperplasia
Seminal Vesicles
Kidney
Neoplasms
Organ Specificity
Polyploidy
Papillomavirus Infections
Fertilization in Vitro
Transgenes
Uterine Cervical Neoplasms
Epithelium
Genes

Keywords

  • Cervical neoplasia
  • HPV-18
  • Preputial gland
  • Seminal vesicle
  • Transgenic mice

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

E6 and E7 expression from the HPV 18 LCR : Development of genital hyperplasia and neoplasia in transgenic mice. / Comerford, Sarah A; Maika, S. D.; Laimins, L. A.; Messing, A.; Elsasser, H. P.; Hammer, Robert E.

In: Oncogene, Vol. 10, No. 3, 1995, p. 587-597.

Research output: Contribution to journalArticle

Comerford, Sarah A ; Maika, S. D. ; Laimins, L. A. ; Messing, A. ; Elsasser, H. P. ; Hammer, Robert E. / E6 and E7 expression from the HPV 18 LCR : Development of genital hyperplasia and neoplasia in transgenic mice. In: Oncogene. 1995 ; Vol. 10, No. 3. pp. 587-597.
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