Early gene response to low-intensity pulsed ultrasound in rat osteoblastic cells

Kotaro Sena, Robert M. Leven, Khurram Mazhar, Dale R. Sumner, Amarjit S. Virdi

Research output: Contribution to journalArticlepeer-review

108 Scopus citations


The aim of the current research was to quantify the changes in gene expression in rat bone marrow derived stromal cells (BMSC) to low intensity pulsed ultrasound (LIPUS) during early time points after the ultrasound application. LIPUS at 1.5 MHz, 30 mW/cm2 was applied to BMSC for a single 20 min treatment. Real-time PCR was carried out to quantify the expression of early response genes and bone differentiation marker genes 0.5, 1, 3, 6 and 12 h after the end of the LIPUS treatment. Compared with the controls, LIPUS treatment resulted in elevated transient expression of early response genes (c-jun, c-myc, COX-2, Egr-1, TSC-22) as well as the bone differentiation marker genes, osteonectin and osteopontin, at 3 h. This induction of early response genes as well as extracellular matrix genes associated with cell proliferation and differentiation may represent the effect of LIPUS to cells of osteoblastic lineage.

Original languageEnglish (US)
Pages (from-to)703-708
Number of pages6
JournalUltrasound in Medicine and Biology
Issue number5
StatePublished - May 2005


  • Bone marrow derived stromal cells
  • Fracture healing
  • Gene expression
  • Low-intensity pulsed ultrasound

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics


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