TY - JOUR
T1 - Early gene response to low-intensity pulsed ultrasound in rat osteoblastic cells
AU - Sena, Kotaro
AU - Leven, Robert M.
AU - Mazhar, Khurram
AU - Sumner, Dale R.
AU - Virdi, Amarjit S.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2005/5
Y1 - 2005/5
N2 - The aim of the current research was to quantify the changes in gene expression in rat bone marrow derived stromal cells (BMSC) to low intensity pulsed ultrasound (LIPUS) during early time points after the ultrasound application. LIPUS at 1.5 MHz, 30 mW/cm2 was applied to BMSC for a single 20 min treatment. Real-time PCR was carried out to quantify the expression of early response genes and bone differentiation marker genes 0.5, 1, 3, 6 and 12 h after the end of the LIPUS treatment. Compared with the controls, LIPUS treatment resulted in elevated transient expression of early response genes (c-jun, c-myc, COX-2, Egr-1, TSC-22) as well as the bone differentiation marker genes, osteonectin and osteopontin, at 3 h. This induction of early response genes as well as extracellular matrix genes associated with cell proliferation and differentiation may represent the effect of LIPUS to cells of osteoblastic lineage.
AB - The aim of the current research was to quantify the changes in gene expression in rat bone marrow derived stromal cells (BMSC) to low intensity pulsed ultrasound (LIPUS) during early time points after the ultrasound application. LIPUS at 1.5 MHz, 30 mW/cm2 was applied to BMSC for a single 20 min treatment. Real-time PCR was carried out to quantify the expression of early response genes and bone differentiation marker genes 0.5, 1, 3, 6 and 12 h after the end of the LIPUS treatment. Compared with the controls, LIPUS treatment resulted in elevated transient expression of early response genes (c-jun, c-myc, COX-2, Egr-1, TSC-22) as well as the bone differentiation marker genes, osteonectin and osteopontin, at 3 h. This induction of early response genes as well as extracellular matrix genes associated with cell proliferation and differentiation may represent the effect of LIPUS to cells of osteoblastic lineage.
KW - Bone marrow derived stromal cells
KW - Fracture healing
KW - Gene expression
KW - Low-intensity pulsed ultrasound
UR - http://www.scopus.com/inward/record.url?scp=18144403178&partnerID=8YFLogxK
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U2 - 10.1016/j.ultrasmedbio.2005.01.013
DO - 10.1016/j.ultrasmedbio.2005.01.013
M3 - Article
C2 - 15866420
AN - SCOPUS:18144403178
SN - 0301-5629
VL - 31
SP - 703
EP - 708
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 5
ER -