Early mitochondrial adaptations in skeletal muscle to diet-induced obesity are strain dependent and determine oxidative stress and energy expenditure but not insulin sensitivity

Sihem Boudina, Sandra Sena, Crystal Sloan, Ali Tebbi, Yong Hwan Han, Brian T. O'Neill, Robert C. Cooksey, Deborah Jones, William L. Holland, Donald A. McClain, E. Dale Abel

Research output: Contribution to journalArticle

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Abstract

This study sought to elucidate the relationshipbetweenskeletal muscle mitochondrial dysfunction, oxidative stress, and insulin resistance in two mouse models with differential susceptibility to diet-induced obesity. We examined the time course of mitochondrial dysfunction and insulin resistance in obesity-prone C57B and obesity-resistant FVB mouse strains in response to high-fat feeding. After 5 wk, impaired insulin-mediated glucose uptake in skeletal muscle developed in both strains in the absence of any impairment in proximal insulin signaling. Impaired mitochondrial oxidative capacity preceded the development of insulin resistant glucose uptake in C57B mice in concert with increased oxidative stress in skeletal muscle. By contrast, mitochondrial uncoupling in FVB mice, which prevented oxidative stress and increased energy expenditure, did not prevent insulin resistant glucose uptake in skeletal muscle. Preventing oxidative stress in C57B mice treated systemically with an antioxidant normalized skeletal muscle mitochondrial function but failed to normalize glucose tolerance and insulin sensitivity. Furthermore, high fat-fed uncoupling protein 3 knockout mice developed increased oxidative stress that did not worsen glucose tolerance. In the evolution of diet-induced obesity and insulin resistance, initial but divergent strain-dependent mitochondrial adaptations modulate oxidative stress and energy expenditure without influencing the onset of impaired insulin-mediated glucose uptake.

Original languageEnglish (US)
Pages (from-to)2677-2688
Number of pages12
JournalEndocrinology
Volume153
Issue number6
DOIs
StatePublished - Jun 2012

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Energy Metabolism
Insulin Resistance
Skeletal Muscle
Oxidative Stress
Obesity
Diet
Glucose
Insulin
Fats
Knockout Mice
Antioxidants
Muscles

ASJC Scopus subject areas

  • Endocrinology

Cite this

Early mitochondrial adaptations in skeletal muscle to diet-induced obesity are strain dependent and determine oxidative stress and energy expenditure but not insulin sensitivity. / Boudina, Sihem; Sena, Sandra; Sloan, Crystal; Tebbi, Ali; Han, Yong Hwan; O'Neill, Brian T.; Cooksey, Robert C.; Jones, Deborah; Holland, William L.; McClain, Donald A.; Abel, E. Dale.

In: Endocrinology, Vol. 153, No. 6, 06.2012, p. 2677-2688.

Research output: Contribution to journalArticle

Boudina, S, Sena, S, Sloan, C, Tebbi, A, Han, YH, O'Neill, BT, Cooksey, RC, Jones, D, Holland, WL, McClain, DA & Abel, ED 2012, 'Early mitochondrial adaptations in skeletal muscle to diet-induced obesity are strain dependent and determine oxidative stress and energy expenditure but not insulin sensitivity', Endocrinology, vol. 153, no. 6, pp. 2677-2688. https://doi.org/10.1210/en.2011-2147
Boudina, Sihem ; Sena, Sandra ; Sloan, Crystal ; Tebbi, Ali ; Han, Yong Hwan ; O'Neill, Brian T. ; Cooksey, Robert C. ; Jones, Deborah ; Holland, William L. ; McClain, Donald A. ; Abel, E. Dale. / Early mitochondrial adaptations in skeletal muscle to diet-induced obesity are strain dependent and determine oxidative stress and energy expenditure but not insulin sensitivity. In: Endocrinology. 2012 ; Vol. 153, No. 6. pp. 2677-2688.
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