Early myocardial dysfunction in streptozotocin-induced diabetic mice: A study using in vivo magnetic resonance imaging (MRI)

Xichun Yu, Yasvir A. Tesiram, Rheal A. Towner, Andrew Abbott, Eugene Patterson, Shijun Huang, Marion W. Garrett, Suresh Chandrasekaran, Satoshi Matsuzaki, Luke I. Szweda, Brian E. Gordon, David C. Kem

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Background: Diabetes is associated with a cardiomyopathy that is independent of coronary artery disease or hypertension. In the present study we used in vivo magnetic resonance imaging (MRI) and echocardiographic techniques to examine and characterize early changes in myocardial function in a mouse model of type 1 diabetes. Methods: Diabetes was induced in 8-week old C57BL/6 mice with two intraperitoneal injections of streptozotocin. The blood glucose levels were maintained at 19-25 mmol/l using intermittent low dosages of long acting insulin glargine. MRI and echocardiography were performed at 4 weeks of diabetes (age of 12 weeks) in diabetic mice and age-matched controls. Results: After 4 weeks of hyperglycemia one marker of mitochondrial function, NADH oxidase activity, was decreased to 50% of control animals. MRI studies of diabetic mice at 4 weeks demonstrated significant deficits in myocardial morphology and functionality including: a decreased left ventricular (LV) wall thickness, an increased LV end-systolic diameter and volume, a diminished LV ejection fraction and cardiac output, a decreased LV circumferential shortening, and decreased LV peak ejection and filling rates. M-mode echocardiographic and Doppler flow studies of diabetic mice at 4 weeks showed a decreased wall thickening and increased E/A ratio, supporting both systolic and diastolic dysfunction. Conclusion: Our study demonstrates that MRI interrogation can identify the onset of diabetic cardiomyopathy in mice with its impaired functional capacity and altered morphology. The MRI technique will lend itself to repetitive study of early changes in cardiac function in small animal models of diabetic cardiomyopathy.

Original languageEnglish (US)
Article number6
JournalCardiovascular Diabetology
Volume6
DOIs
StatePublished - Mar 9 2007

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Streptozocin
Magnetic Resonance Imaging
Diabetic Cardiomyopathies
Stroke Volume
Long-Acting Insulin
Intraperitoneal Injections
Type 1 Diabetes Mellitus
Cardiomyopathies
Inbred C57BL Mouse
Hyperglycemia
Cardiac Output
Echocardiography
Blood Glucose
Coronary Artery Disease
Animal Models
Hypertension

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Cardiology and Cardiovascular Medicine

Cite this

Early myocardial dysfunction in streptozotocin-induced diabetic mice : A study using in vivo magnetic resonance imaging (MRI). / Yu, Xichun; Tesiram, Yasvir A.; Towner, Rheal A.; Abbott, Andrew; Patterson, Eugene; Huang, Shijun; Garrett, Marion W.; Chandrasekaran, Suresh; Matsuzaki, Satoshi; Szweda, Luke I.; Gordon, Brian E.; Kem, David C.

In: Cardiovascular Diabetology, Vol. 6, 6, 09.03.2007.

Research output: Contribution to journalArticle

Yu, X, Tesiram, YA, Towner, RA, Abbott, A, Patterson, E, Huang, S, Garrett, MW, Chandrasekaran, S, Matsuzaki, S, Szweda, LI, Gordon, BE & Kem, DC 2007, 'Early myocardial dysfunction in streptozotocin-induced diabetic mice: A study using in vivo magnetic resonance imaging (MRI)', Cardiovascular Diabetology, vol. 6, 6. https://doi.org/10.1186/1475-2840-6-6
Yu, Xichun ; Tesiram, Yasvir A. ; Towner, Rheal A. ; Abbott, Andrew ; Patterson, Eugene ; Huang, Shijun ; Garrett, Marion W. ; Chandrasekaran, Suresh ; Matsuzaki, Satoshi ; Szweda, Luke I. ; Gordon, Brian E. ; Kem, David C. / Early myocardial dysfunction in streptozotocin-induced diabetic mice : A study using in vivo magnetic resonance imaging (MRI). In: Cardiovascular Diabetology. 2007 ; Vol. 6.
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AU - Towner, Rheal A.

AU - Abbott, Andrew

AU - Patterson, Eugene

AU - Huang, Shijun

AU - Garrett, Marion W.

AU - Chandrasekaran, Suresh

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AU - Gordon, Brian E.

AU - Kem, David C.

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N2 - Background: Diabetes is associated with a cardiomyopathy that is independent of coronary artery disease or hypertension. In the present study we used in vivo magnetic resonance imaging (MRI) and echocardiographic techniques to examine and characterize early changes in myocardial function in a mouse model of type 1 diabetes. Methods: Diabetes was induced in 8-week old C57BL/6 mice with two intraperitoneal injections of streptozotocin. The blood glucose levels were maintained at 19-25 mmol/l using intermittent low dosages of long acting insulin glargine. MRI and echocardiography were performed at 4 weeks of diabetes (age of 12 weeks) in diabetic mice and age-matched controls. Results: After 4 weeks of hyperglycemia one marker of mitochondrial function, NADH oxidase activity, was decreased to 50% of control animals. MRI studies of diabetic mice at 4 weeks demonstrated significant deficits in myocardial morphology and functionality including: a decreased left ventricular (LV) wall thickness, an increased LV end-systolic diameter and volume, a diminished LV ejection fraction and cardiac output, a decreased LV circumferential shortening, and decreased LV peak ejection and filling rates. M-mode echocardiographic and Doppler flow studies of diabetic mice at 4 weeks showed a decreased wall thickening and increased E/A ratio, supporting both systolic and diastolic dysfunction. Conclusion: Our study demonstrates that MRI interrogation can identify the onset of diabetic cardiomyopathy in mice with its impaired functional capacity and altered morphology. The MRI technique will lend itself to repetitive study of early changes in cardiac function in small animal models of diabetic cardiomyopathy.

AB - Background: Diabetes is associated with a cardiomyopathy that is independent of coronary artery disease or hypertension. In the present study we used in vivo magnetic resonance imaging (MRI) and echocardiographic techniques to examine and characterize early changes in myocardial function in a mouse model of type 1 diabetes. Methods: Diabetes was induced in 8-week old C57BL/6 mice with two intraperitoneal injections of streptozotocin. The blood glucose levels were maintained at 19-25 mmol/l using intermittent low dosages of long acting insulin glargine. MRI and echocardiography were performed at 4 weeks of diabetes (age of 12 weeks) in diabetic mice and age-matched controls. Results: After 4 weeks of hyperglycemia one marker of mitochondrial function, NADH oxidase activity, was decreased to 50% of control animals. MRI studies of diabetic mice at 4 weeks demonstrated significant deficits in myocardial morphology and functionality including: a decreased left ventricular (LV) wall thickness, an increased LV end-systolic diameter and volume, a diminished LV ejection fraction and cardiac output, a decreased LV circumferential shortening, and decreased LV peak ejection and filling rates. M-mode echocardiographic and Doppler flow studies of diabetic mice at 4 weeks showed a decreased wall thickening and increased E/A ratio, supporting both systolic and diastolic dysfunction. Conclusion: Our study demonstrates that MRI interrogation can identify the onset of diabetic cardiomyopathy in mice with its impaired functional capacity and altered morphology. The MRI technique will lend itself to repetitive study of early changes in cardiac function in small animal models of diabetic cardiomyopathy.

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