Ectopic expression of guanylyl cyclase C in CD34+ progenitor cells in peripheral blood

T. A. Fava, R. Desnoyers, S. Schulz, J. Park, D. Weinberg, E. Mitchell, S. A. Waldman

Research output: Contribution to journalArticle

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Abstract

Purpose: To examine the utility of guanylyl cyclase C (GC-C)-specific nested reverse transcriptase polymerase chain reaction (RT-PCR) to detect circulating tumor cells in patients with colorectal cancer. Patients and Methods: Peripheral-blood mononuclear cells from 24 patients with Dukes' stage D colorectal cancer were analyzed by GC-C-specific nested RT-PCR using 1 μg of total RNA. Peripheral-blood mononuclear cells from 20 healthy volunteers served as controls. Additionally, peripheral-blood CD34+ progenitor cells were assayed for the expression of both GC-C and other epithelial cell-specific markers. Results: GC-C mRNA was detected in blood mononuclear cells from all 24 patients with colorectal cancer and all healthy volunteers. These unexpected positive results reflected low-level ectopic transcription of GC-C in CD34+ progenitor cells. Moreover, CD34+ progenitor cells expressed other epithelial cell-specific markers, including prostate-specific antigen, prostate-specific membrane antigen, carcinoembryonic antigen, CK-19, CK-20, mucin 1, and GA733.2. Limiting the quantity of mononuclear cell total RNA analyzed to ≤ 0.8 μg eliminated detection of GC-C and other tissue-specific transcripts in blood of healthy volunteers. However, under the same conditions, GC-C mRNA was detected in mononuclear cells from all 24 patients with metastatic colorectal cancer. Using 0.5 μg of total RNA and GC-C-specific primers, nested RT-PCR detected a single human colon carcinoma cell (approximately 20 to 200 GC-C transcripts/cell) in 106 to 107 mononuclear blood cells. Conclusion: These data suggest that GC-C may be useful for detecting circulating colorectal cancer cells. They also demonstrate that CD34+ cells are a source of ectopically expressed epithelial cell-specific markers and that CD34+ cells may contribute to the high false-positive rate generally observed when those markers are used to detect rare circulating metastatic cancer cells by RT-PCR.

Original languageEnglish (US)
Pages (from-to)3951-3959
Number of pages9
JournalJournal of Clinical Oncology
Volume19
Issue number19
StatePublished - Oct 1 2001

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Stem Cells
Colorectal Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
Blood Cells
Healthy Volunteers
Epithelial Cells
RNA
enterotoxin receptor
Ectopic Gene Expression
Circulating Neoplastic Cells
Mucin-1
Messenger RNA
Carcinoembryonic Antigen
Prostate-Specific Antigen
Colon
Carcinoma
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Fava, T. A., Desnoyers, R., Schulz, S., Park, J., Weinberg, D., Mitchell, E., & Waldman, S. A. (2001). Ectopic expression of guanylyl cyclase C in CD34+ progenitor cells in peripheral blood. Journal of Clinical Oncology, 19(19), 3951-3959.

Ectopic expression of guanylyl cyclase C in CD34+ progenitor cells in peripheral blood. / Fava, T. A.; Desnoyers, R.; Schulz, S.; Park, J.; Weinberg, D.; Mitchell, E.; Waldman, S. A.

In: Journal of Clinical Oncology, Vol. 19, No. 19, 01.10.2001, p. 3951-3959.

Research output: Contribution to journalArticle

Fava, TA, Desnoyers, R, Schulz, S, Park, J, Weinberg, D, Mitchell, E & Waldman, SA 2001, 'Ectopic expression of guanylyl cyclase C in CD34+ progenitor cells in peripheral blood', Journal of Clinical Oncology, vol. 19, no. 19, pp. 3951-3959.
Fava TA, Desnoyers R, Schulz S, Park J, Weinberg D, Mitchell E et al. Ectopic expression of guanylyl cyclase C in CD34+ progenitor cells in peripheral blood. Journal of Clinical Oncology. 2001 Oct 1;19(19):3951-3959.
Fava, T. A. ; Desnoyers, R. ; Schulz, S. ; Park, J. ; Weinberg, D. ; Mitchell, E. ; Waldman, S. A. / Ectopic expression of guanylyl cyclase C in CD34+ progenitor cells in peripheral blood. In: Journal of Clinical Oncology. 2001 ; Vol. 19, No. 19. pp. 3951-3959.
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abstract = "Purpose: To examine the utility of guanylyl cyclase C (GC-C)-specific nested reverse transcriptase polymerase chain reaction (RT-PCR) to detect circulating tumor cells in patients with colorectal cancer. Patients and Methods: Peripheral-blood mononuclear cells from 24 patients with Dukes' stage D colorectal cancer were analyzed by GC-C-specific nested RT-PCR using 1 μg of total RNA. Peripheral-blood mononuclear cells from 20 healthy volunteers served as controls. Additionally, peripheral-blood CD34+ progenitor cells were assayed for the expression of both GC-C and other epithelial cell-specific markers. Results: GC-C mRNA was detected in blood mononuclear cells from all 24 patients with colorectal cancer and all healthy volunteers. These unexpected positive results reflected low-level ectopic transcription of GC-C in CD34+ progenitor cells. Moreover, CD34+ progenitor cells expressed other epithelial cell-specific markers, including prostate-specific antigen, prostate-specific membrane antigen, carcinoembryonic antigen, CK-19, CK-20, mucin 1, and GA733.2. Limiting the quantity of mononuclear cell total RNA analyzed to ≤ 0.8 μg eliminated detection of GC-C and other tissue-specific transcripts in blood of healthy volunteers. However, under the same conditions, GC-C mRNA was detected in mononuclear cells from all 24 patients with metastatic colorectal cancer. Using 0.5 μg of total RNA and GC-C-specific primers, nested RT-PCR detected a single human colon carcinoma cell (approximately 20 to 200 GC-C transcripts/cell) in 106 to 107 mononuclear blood cells. Conclusion: These data suggest that GC-C may be useful for detecting circulating colorectal cancer cells. They also demonstrate that CD34+ cells are a source of ectopically expressed epithelial cell-specific markers and that CD34+ cells may contribute to the high false-positive rate generally observed when those markers are used to detect rare circulating metastatic cancer cells by RT-PCR.",
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AU - Desnoyers, R.

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AU - Weinberg, D.

AU - Mitchell, E.

AU - Waldman, S. A.

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N2 - Purpose: To examine the utility of guanylyl cyclase C (GC-C)-specific nested reverse transcriptase polymerase chain reaction (RT-PCR) to detect circulating tumor cells in patients with colorectal cancer. Patients and Methods: Peripheral-blood mononuclear cells from 24 patients with Dukes' stage D colorectal cancer were analyzed by GC-C-specific nested RT-PCR using 1 μg of total RNA. Peripheral-blood mononuclear cells from 20 healthy volunteers served as controls. Additionally, peripheral-blood CD34+ progenitor cells were assayed for the expression of both GC-C and other epithelial cell-specific markers. Results: GC-C mRNA was detected in blood mononuclear cells from all 24 patients with colorectal cancer and all healthy volunteers. These unexpected positive results reflected low-level ectopic transcription of GC-C in CD34+ progenitor cells. Moreover, CD34+ progenitor cells expressed other epithelial cell-specific markers, including prostate-specific antigen, prostate-specific membrane antigen, carcinoembryonic antigen, CK-19, CK-20, mucin 1, and GA733.2. Limiting the quantity of mononuclear cell total RNA analyzed to ≤ 0.8 μg eliminated detection of GC-C and other tissue-specific transcripts in blood of healthy volunteers. However, under the same conditions, GC-C mRNA was detected in mononuclear cells from all 24 patients with metastatic colorectal cancer. Using 0.5 μg of total RNA and GC-C-specific primers, nested RT-PCR detected a single human colon carcinoma cell (approximately 20 to 200 GC-C transcripts/cell) in 106 to 107 mononuclear blood cells. Conclusion: These data suggest that GC-C may be useful for detecting circulating colorectal cancer cells. They also demonstrate that CD34+ cells are a source of ectopically expressed epithelial cell-specific markers and that CD34+ cells may contribute to the high false-positive rate generally observed when those markers are used to detect rare circulating metastatic cancer cells by RT-PCR.

AB - Purpose: To examine the utility of guanylyl cyclase C (GC-C)-specific nested reverse transcriptase polymerase chain reaction (RT-PCR) to detect circulating tumor cells in patients with colorectal cancer. Patients and Methods: Peripheral-blood mononuclear cells from 24 patients with Dukes' stage D colorectal cancer were analyzed by GC-C-specific nested RT-PCR using 1 μg of total RNA. Peripheral-blood mononuclear cells from 20 healthy volunteers served as controls. Additionally, peripheral-blood CD34+ progenitor cells were assayed for the expression of both GC-C and other epithelial cell-specific markers. Results: GC-C mRNA was detected in blood mononuclear cells from all 24 patients with colorectal cancer and all healthy volunteers. These unexpected positive results reflected low-level ectopic transcription of GC-C in CD34+ progenitor cells. Moreover, CD34+ progenitor cells expressed other epithelial cell-specific markers, including prostate-specific antigen, prostate-specific membrane antigen, carcinoembryonic antigen, CK-19, CK-20, mucin 1, and GA733.2. Limiting the quantity of mononuclear cell total RNA analyzed to ≤ 0.8 μg eliminated detection of GC-C and other tissue-specific transcripts in blood of healthy volunteers. However, under the same conditions, GC-C mRNA was detected in mononuclear cells from all 24 patients with metastatic colorectal cancer. Using 0.5 μg of total RNA and GC-C-specific primers, nested RT-PCR detected a single human colon carcinoma cell (approximately 20 to 200 GC-C transcripts/cell) in 106 to 107 mononuclear blood cells. Conclusion: These data suggest that GC-C may be useful for detecting circulating colorectal cancer cells. They also demonstrate that CD34+ cells are a source of ectopically expressed epithelial cell-specific markers and that CD34+ cells may contribute to the high false-positive rate generally observed when those markers are used to detect rare circulating metastatic cancer cells by RT-PCR.

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