Ectopic expression of X-linked lymphocyte-regulated protein pM1 renders tumor cells resistant to antitumor immunity

Tae Heung Kang, Kyung Hee Noh, Jin Hee Kim, Hyun Cheol Bae, Ken Y. Lin, Archana Monie, Sara I. Pai, Chien Fu Hung, T. C. Wu, Tae Woo Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Tumor immune escape is a major obstacle in cancer immunotherapy, but the mechanisms involved remain poorly understood. We have previously developed an immune evasion tumor model using an in vivo immune selection strategy and revealed Akt-mediated immune resistance to antitumor immunity induced by various cancer immunotherapeutic agents. In the current study, we used microarray gene analysis to identify an Akt-activating candidate molecule overexpressed in immune-resistant tumors compared with parental tumors. X-linked lymphocyte-regulated protein pM1 (XLR) gene was the most upregulated in immune-resistant tumors compared with parental tumor cells. Furthermore, the retroviral transduction of XLR in parental tumor cells led to activation of Akt, resulting in upregulation of antiapoptotic proteins and the induction of immune resistance phenotype in parental tumor cells. In addition, we found that transduction of parental tumor cells with other homologous genes from the mouse XLR family, such as synaptonemal complex protein 3 (SCP3) and XLR-related, meiosis-regulated protein (XMR) and its human counterpart of SCP3 (hSCP3), also led to activation of Akt, resulting in the upregulation of antiapoptotic proteins and induction of immune resistance phenotype. Importantly, characterization of a panel of human cervical cancers revealed relatively higher expression levels of hSCP3 in human cervical cancer tissue compared with normal cervical tissue. Thus, our data indicate that ectopic expression of XLR and its homologues in tumor cells represents a potentially important mechanism for tumor immune evasion and serves as a promising molecular target for cancer immunotherapy.

Original languageEnglish (US)
Pages (from-to)3062-3070
Number of pages9
JournalCancer Research
Volume70
Issue number8
DOIs
StatePublished - Apr 15 2010

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Immunity
Lymphocytes
Neoplasms
Proteins
Tumor Escape
Uterine Cervical Neoplasms
Immunotherapy
Ectopic Gene Expression
Up-Regulation
Synaptonemal Complex
Genes
Phenotype
Meiosis
Microarray Analysis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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Ectopic expression of X-linked lymphocyte-regulated protein pM1 renders tumor cells resistant to antitumor immunity. / Kang, Tae Heung; Noh, Kyung Hee; Kim, Jin Hee; Bae, Hyun Cheol; Lin, Ken Y.; Monie, Archana; Pai, Sara I.; Hung, Chien Fu; Wu, T. C.; Kim, Tae Woo.

In: Cancer Research, Vol. 70, No. 8, 15.04.2010, p. 3062-3070.

Research output: Contribution to journalArticle

Kang, TH, Noh, KH, Kim, JH, Bae, HC, Lin, KY, Monie, A, Pai, SI, Hung, CF, Wu, TC & Kim, TW 2010, 'Ectopic expression of X-linked lymphocyte-regulated protein pM1 renders tumor cells resistant to antitumor immunity', Cancer Research, vol. 70, no. 8, pp. 3062-3070. https://doi.org/10.1158/0008-5472.CAN-09-3856
Kang, Tae Heung ; Noh, Kyung Hee ; Kim, Jin Hee ; Bae, Hyun Cheol ; Lin, Ken Y. ; Monie, Archana ; Pai, Sara I. ; Hung, Chien Fu ; Wu, T. C. ; Kim, Tae Woo. / Ectopic expression of X-linked lymphocyte-regulated protein pM1 renders tumor cells resistant to antitumor immunity. In: Cancer Research. 2010 ; Vol. 70, No. 8. pp. 3062-3070.
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