Effect of 17-α hydroxyprogesterone caproate on the production of tumor necrosis factor-α and the expression of cyclooxygenase-2 in lipopolysaccharide-treated gravid human myometrial explants

S. Patel, A. Li, T. M. Goodwin, M. Brower, M. Blitz, P. Minoo, J. C. Felix, R. H. Lee

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Objective: To determine whether 17-α hydroxyprogesterone (17-OHPC) alters tumor necrosis factor-α (TNF-α) production and the expression of cyclooxygenase type 2 (COX-2) in myometrium exposed to lipopolysaccharide (LPS). Study Design: Lower segment myometrial biopsies were obtained from non-laboring patients at term. Tissues were cultured in serum-free media with 17-OHPC (1 M) and LPS (1 g/ml), either alone or in combination. At 24 h, the production of tumor necrosis factor-α (TNF-α) and the expression of COX-2 was determined using enzyme linked immunosorbent assay and real-time (RT-PCR). Statistical analysis was performed using non-parametric testing. A P-value of 0.05 was considered significant.Result:17-OHPC had no effect on TNF-α production and COX-2 expression when compared with untreated myometrial explants (P0.61 and P0.95). LPS induced production of TNF-α (P0.03) and expression of COX-2 (P0.02). Treatment with 17-OHPC did not block LPS-induced TNF-α production (P0.37) or COX-2 expression (P0.12).Conclusion:In this pilot study, 17-OHPC did not affect the production of TNF-α or COX-2 expression in human myometrium.

Original languageEnglish (US)
Pages (from-to)584-589
Number of pages6
JournalJournal of Perinatology
Issue number9
StatePublished - Sep 1 2010


ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

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