Effect of amino acid substitutions in the signal peptide cleavage region on the processing of escherichia coli alkaline phosphatase

A. V. Kajava, A. I. Karamyshev, Z. N. Karaniysheva, V. N. Ksenzenko, A. Kalinin, M. A. Nesmeyanova

Research output: Contribution to journalArticle

Abstract

A wide range (69) of mutant Kt,chtnchia coh alkaline phosphatases (PhoA) with various single ami no acid substitutions ai positions from > îo \-\ of the signal peptide (SP) were obtained to .study tIJP protein processing as a function of the cleavage .site sequence. Amber suppressor mutagenesis was used included: (i) introduction of amber mutations into the corresponding positions, and (ii) expression of the mutant, genes in K.roh strains producing amber-suppressor tRNAs specific to Her, Gin. Tyr. Leu, Ala. Glu, Plie. (îly. His, Cys, Lys and Pro. The processing of the mutant was assayed in pulse chase experiments in vivo. The results gave a new expérimental support for the (-3, -1 )-rule. The data also reveals a higher amino acids conservation at -1 position of Gram-negative bacteriaas compared with the eukaryotir organisms. The efficient processing was provided by medium-size residues at position .r). This suggests that the size of residues not only at positions -1 and II, as it was revealed earlier, but also at position -5 effects the processing rate. Hased on the stereochemical analysis we suggest a conformation of the 5 to 1 region of SP in the SPase binding pocket thai is consistent with the mutagenesis and statistical data. I'hr authors ijrattfnlly acknowlfdgf support by fhf Swint \dtirmal Scirnrt Foundation (grant 7Sl:I'JOJMjQ!.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997

Fingerprint

amino acid substitution
Amino Acid Substitution
Protein Sorting Signals
signal peptide
Mutagenesis
Escherichia coli
Alkaline Phosphatase
alkaline phosphatase
Substitution reactions
amber
Amber
Amino Acids
mutagenesis
mutants
Organized Financing
Processing
Transfer RNA
nonsense mutation
Gene Expression
Mutation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Kajava, A. V., Karamyshev, A. I., Karaniysheva, Z. N., Ksenzenko, V. N., Kalinin, A., & Nesmeyanova, M. A. (1997). Effect of amino acid substitutions in the signal peptide cleavage region on the processing of escherichia coli alkaline phosphatase. FASEB Journal, 11(9).

Effect of amino acid substitutions in the signal peptide cleavage region on the processing of escherichia coli alkaline phosphatase. / Kajava, A. V.; Karamyshev, A. I.; Karaniysheva, Z. N.; Ksenzenko, V. N.; Kalinin, A.; Nesmeyanova, M. A.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

Kajava, AV, Karamyshev, AI, Karaniysheva, ZN, Ksenzenko, VN, Kalinin, A & Nesmeyanova, MA 1997, 'Effect of amino acid substitutions in the signal peptide cleavage region on the processing of escherichia coli alkaline phosphatase', FASEB Journal, vol. 11, no. 9.
Kajava AV, Karamyshev AI, Karaniysheva ZN, Ksenzenko VN, Kalinin A, Nesmeyanova MA. Effect of amino acid substitutions in the signal peptide cleavage region on the processing of escherichia coli alkaline phosphatase. FASEB Journal. 1997;11(9).
Kajava, A. V. ; Karamyshev, A. I. ; Karaniysheva, Z. N. ; Ksenzenko, V. N. ; Kalinin, A. ; Nesmeyanova, M. A. / Effect of amino acid substitutions in the signal peptide cleavage region on the processing of escherichia coli alkaline phosphatase. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
@article{fd9490a859d64e12b685c0f243c6d8f2,
title = "Effect of amino acid substitutions in the signal peptide cleavage region on the processing of escherichia coli alkaline phosphatase",
abstract = "A wide range (69) of mutant Kt,chtnchia coh alkaline phosphatases (PhoA) with various single ami no acid substitutions ai positions from > {\^i}o \-\ of the signal peptide (SP) were obtained to .study tIJP protein processing as a function of the cleavage .site sequence. Amber suppressor mutagenesis was used included: (i) introduction of amber mutations into the corresponding positions, and (ii) expression of the mutant, genes in K.roh strains producing amber-suppressor tRNAs specific to Her, Gin. Tyr. Leu, Ala. Glu, Plie. ({\^i}ly. His, Cys, Lys and Pro. The processing of the mutant was assayed in pulse chase experiments in vivo. The results gave a new exp{\'e}rimental support for the (-3, -1 )-rule. The data also reveals a higher amino acids conservation at -1 position of Gram-negative bacteriaas compared with the eukaryotir organisms. The efficient processing was provided by medium-size residues at position .r). This suggests that the size of residues not only at positions -1 and II, as it was revealed earlier, but also at position -5 effects the processing rate. Hased on the stereochemical analysis we suggest a conformation of the 5 to 1 region of SP in the SPase binding pocket thai is consistent with the mutagenesis and statistical data. I'hr authors ijrattfnlly acknowlfdgf support by fhf Swint \dtirmal Scirnrt Foundation (grant 7Sl:I'JOJMjQ!.",
author = "Kajava, {A. V.} and Karamyshev, {A. I.} and Karaniysheva, {Z. N.} and Ksenzenko, {V. N.} and A. Kalinin and Nesmeyanova, {M. A.}",
year = "1997",
language = "English (US)",
volume = "11",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "9",

}

TY - JOUR

T1 - Effect of amino acid substitutions in the signal peptide cleavage region on the processing of escherichia coli alkaline phosphatase

AU - Kajava, A. V.

AU - Karamyshev, A. I.

AU - Karaniysheva, Z. N.

AU - Ksenzenko, V. N.

AU - Kalinin, A.

AU - Nesmeyanova, M. A.

PY - 1997

Y1 - 1997

N2 - A wide range (69) of mutant Kt,chtnchia coh alkaline phosphatases (PhoA) with various single ami no acid substitutions ai positions from > îo \-\ of the signal peptide (SP) were obtained to .study tIJP protein processing as a function of the cleavage .site sequence. Amber suppressor mutagenesis was used included: (i) introduction of amber mutations into the corresponding positions, and (ii) expression of the mutant, genes in K.roh strains producing amber-suppressor tRNAs specific to Her, Gin. Tyr. Leu, Ala. Glu, Plie. (îly. His, Cys, Lys and Pro. The processing of the mutant was assayed in pulse chase experiments in vivo. The results gave a new expérimental support for the (-3, -1 )-rule. The data also reveals a higher amino acids conservation at -1 position of Gram-negative bacteriaas compared with the eukaryotir organisms. The efficient processing was provided by medium-size residues at position .r). This suggests that the size of residues not only at positions -1 and II, as it was revealed earlier, but also at position -5 effects the processing rate. Hased on the stereochemical analysis we suggest a conformation of the 5 to 1 region of SP in the SPase binding pocket thai is consistent with the mutagenesis and statistical data. I'hr authors ijrattfnlly acknowlfdgf support by fhf Swint \dtirmal Scirnrt Foundation (grant 7Sl:I'JOJMjQ!.

AB - A wide range (69) of mutant Kt,chtnchia coh alkaline phosphatases (PhoA) with various single ami no acid substitutions ai positions from > îo \-\ of the signal peptide (SP) were obtained to .study tIJP protein processing as a function of the cleavage .site sequence. Amber suppressor mutagenesis was used included: (i) introduction of amber mutations into the corresponding positions, and (ii) expression of the mutant, genes in K.roh strains producing amber-suppressor tRNAs specific to Her, Gin. Tyr. Leu, Ala. Glu, Plie. (îly. His, Cys, Lys and Pro. The processing of the mutant was assayed in pulse chase experiments in vivo. The results gave a new expérimental support for the (-3, -1 )-rule. The data also reveals a higher amino acids conservation at -1 position of Gram-negative bacteriaas compared with the eukaryotir organisms. The efficient processing was provided by medium-size residues at position .r). This suggests that the size of residues not only at positions -1 and II, as it was revealed earlier, but also at position -5 effects the processing rate. Hased on the stereochemical analysis we suggest a conformation of the 5 to 1 region of SP in the SPase binding pocket thai is consistent with the mutagenesis and statistical data. I'hr authors ijrattfnlly acknowlfdgf support by fhf Swint \dtirmal Scirnrt Foundation (grant 7Sl:I'JOJMjQ!.

UR - http://www.scopus.com/inward/record.url?scp=33750133867&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750133867&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33750133867

VL - 11

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 9

ER -