Effect of angiotensin II on human luteinized granulosa cells

W. E. Rainey, I. M. Bird, W. Byrd, B. R. Carr

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Objective: To determine if human luteinized granulosa cells (GCs) exhibit angiotensin II (AII) receptors and if AII affects GC steroidogenesis. Design: Luteinized GCs were either treated immediately or maintained in culture for up to 6 days before use. The presence of functional AII receptors was evaluated using the following criteria: [1] receptor binding of 125I- labeled AII; [2] changes in inositol phosphate production; [3] effects on progesterone (P) production; and [4] effects on cyclic adenosine 3':5' monophosphate (cAMP) production. Results: No specific binding of 125I- labeled AII was observed using human luteinized GCs in culture. Angiotensin II did not alter the formation of [3H] inositol phosphates, nor did it alter the release of P from freshly isolated cells or cells that had been in culture for 5 days. Angiotensin II was also unable to alter basal or gonadotropin-stimulated cAMP production. These data suggest that human luteinized GCs exhibit little or no type 1 or type 2 AII receptors. Conclusion: This observation is in contrast to reports in rat granulosa that exhibit AII receptors. The reason for a lack of AII receptor expression on human luteinized GCs is unknown but could be because of luteal phase down regulation with gonadotropin-releasing hormone agonist or the subsequent stimulation with gonadotropins in these women before follicular stimulation.

Original languageEnglish (US)
Pages (from-to)143-147
Number of pages5
JournalFertility and Sterility
Volume59
Issue number1
StatePublished - 1993

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Granulosa Cells
Angiotensin II
Angiotensin Receptors
Inositol Phosphates
Gonadotropins
Adenosine
Angiotensin Type 2 Receptor
Luteal Phase
Gonadotropin-Releasing Hormone
Progesterone
Down-Regulation
Cell Culture Techniques

Keywords

  • angiotensin II
  • cell culture
  • Human luteinized granulosa

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Effect of angiotensin II on human luteinized granulosa cells. / Rainey, W. E.; Bird, I. M.; Byrd, W.; Carr, B. R.

In: Fertility and Sterility, Vol. 59, No. 1, 1993, p. 143-147.

Research output: Contribution to journalArticle

Rainey, WE, Bird, IM, Byrd, W & Carr, BR 1993, 'Effect of angiotensin II on human luteinized granulosa cells', Fertility and Sterility, vol. 59, no. 1, pp. 143-147.
Rainey, W. E. ; Bird, I. M. ; Byrd, W. ; Carr, B. R. / Effect of angiotensin II on human luteinized granulosa cells. In: Fertility and Sterility. 1993 ; Vol. 59, No. 1. pp. 143-147.
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