Effect of caffeine on the expression of a major X-ray-induced protein in human tumor cells

E. N. Hughes, D. A. Boothman

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We have examined the effect of caffeine on the concomitant processes of the repair of potentially lethal damage (PLD) and the synthesis of X-ray-induced proteins in the human malignant melanoma cell line, Ul-Mel. Caffeine administered at a dose of 5 mM after X radiation not only inhibited PLD repair but also markedly reduced the level of XIP269, a major X-ray-induced protein whose expression has been shown to correlate with the capacity to repair PLD. The expression of the vast majority of other cellular proteins, including seven other X-ray-induced proteins, remained unchanged following caffeine treatment. A possible role for XIP269 in cell cycle delay following DNA damage by X irradiation is discussed.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalRadiation research
Volume125
Issue number3
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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