Effect of chemical modifications on modulation of gene expression by duplex antigene RNAs that are complementary to non-coding transcripts at gene promoters

Jonathan K. Watts, Dongbo Yu, Klaus Charisse, Christophe Montaillier, Pierre Potier, Muthiah Manoharan, David R. Corey

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Antigene RNAs (agRNAs) are small RNA duplexes that target non-coding transcripts rather than mRNA and specifically suppress or activate gene expression in a sequence-dependent manner. For many applications in vivo, it is likely that agRNAs will require chemical modification. We have synthesized agRNAs that contain different classes of chemical modification and have tested their ability to modulate expression of the human progesterone receptor gene. We find that both silencing and activating agRNAs can retain activity after modification. Both guide and passenger strands can be modified and functional agRNAs can contain 2′F-RNA, 2′OMe-RNA, and locked nucleic acid substitutions, or combinations of multiple modifications. The mechanism of agRNA activity appears to be maintained after chemical modification: both native and modified agRNAs modulate recruitment of RNA polymerase II, have the same effect on promoter-derived antisense transcripts, and must be double-stranded. These data demonstrate that agRNA activity is compatible with a wide range of chemical modifications and may facilitate in vivo applications.

Original languageEnglish (US)
Article numbergkq258
Pages (from-to)5242-5259
Number of pages18
JournalNucleic acids research
Volume38
Issue number15
DOIs
StatePublished - Apr 19 2010

ASJC Scopus subject areas

  • Genetics

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