Effect of circulating fibronectin on stimulation of leukocyte oxygen consumption and serum opsonizing function in burned patients

M. K. Dobke, G. Pearson, C. Roberts, B. Germany, E. Heck, B. S. Masters, C. R. Baxter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In a study of 27 thermally burned patients (mean TBSA, 58%; range 32-96%) serum fibronectin levels were decreased with parallel decreased oxygen consumption of stimulated peripheral blood phagocytes and decreased EGTA-blocked burn serum opsonizing activity which correlated with serum fibronectin changes postburn. Normal and burn sera fibronectin content also correlated with the opsonizing times for zymosan and Staphylococcus aureus but not for Enterobacteriaceae. Although in vivo 14 cases showed circulating fibronectin 140 μg/ml or lower and a marked decrease in Staphylococcus aureus opsonization, only two patients from this group revealed positive Staphylococcus aureus blood cultures and serum fibronectin levels were higher in patients with Staphylococcus aureus sepsis than in patients with Enterobacteriaceae sepsis. Supplementary experiments on leukocyte oxidative response after zymosan stimulation in normal, fibronectin-depleted, and fibronectin-reconstituted serum demonstrated that the lag period of oxygen burst is a fibronectin-dependent reaction.

Original languageEnglish (US)
Pages (from-to)882-890
Number of pages9
JournalJournal of Trauma
Volume23
Issue number10
StatePublished - 1983

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Fibronectins
Oxygen Consumption
Leukocytes
Serum
Staphylococcus aureus
Zymosan
Enterobacteriaceae
Sepsis
Egtazic Acid
Phagocytes
Oxygen

ASJC Scopus subject areas

  • Surgery

Cite this

Dobke, M. K., Pearson, G., Roberts, C., Germany, B., Heck, E., Masters, B. S., & Baxter, C. R. (1983). Effect of circulating fibronectin on stimulation of leukocyte oxygen consumption and serum opsonizing function in burned patients. Journal of Trauma, 23(10), 882-890.

Effect of circulating fibronectin on stimulation of leukocyte oxygen consumption and serum opsonizing function in burned patients. / Dobke, M. K.; Pearson, G.; Roberts, C.; Germany, B.; Heck, E.; Masters, B. S.; Baxter, C. R.

In: Journal of Trauma, Vol. 23, No. 10, 1983, p. 882-890.

Research output: Contribution to journalArticle

Dobke, MK, Pearson, G, Roberts, C, Germany, B, Heck, E, Masters, BS & Baxter, CR 1983, 'Effect of circulating fibronectin on stimulation of leukocyte oxygen consumption and serum opsonizing function in burned patients', Journal of Trauma, vol. 23, no. 10, pp. 882-890.
Dobke, M. K. ; Pearson, G. ; Roberts, C. ; Germany, B. ; Heck, E. ; Masters, B. S. ; Baxter, C. R. / Effect of circulating fibronectin on stimulation of leukocyte oxygen consumption and serum opsonizing function in burned patients. In: Journal of Trauma. 1983 ; Vol. 23, No. 10. pp. 882-890.
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