Effect of contact lens preservatives on Acanthamoeba

R. E. Silvany, J. M. Dougherty, James P McCulley

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Single preservatives used in contact lens solutions were evaluated for their effectiveness in killing Acanthamoeba castellanii and Acanthamoeba polyphaga trophozoites and cysts. Preservatives were tested against amoebae at intervals varying from 30 minutes to 24 hours. The preservatives were tested with axenically and nonaxenically grown organisms. Chlorhexidine (0.001% and 0.005%), polyaminopropyl biguanide (0.0015%), benzalkonium chloride (0.001% and 0.004%), and hydrogen peroxide (3%) were very effective preservatives. Lower concentrations of these same preservatives were less effective. Thimerosal (0.001% and 0.004%), sorbic acid (0.1%), potassium sorbate (0.13%), EDTA (0.1%), polyaminopropyl biguanide (0.00005%), and polyquaternium-1 (0.001%) were not effective as tested. However, thimerosal 0.004% when combined in solution with EDTA was effective. Preservatives were more effective when tested against axenically prepared organisms than when tested against co-cultured organisms.

Original languageEnglish (US)
Pages (from-to)854-857
Number of pages4
JournalOphthalmology
Volume98
Issue number6
StatePublished - 1991

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Sorbic Acid
Thimerosal
Acanthamoeba
Contact Lenses
Edetic Acid
Contact Lens Solutions
Acanthamoeba castellanii
Benzalkonium Compounds
Trophozoites
Amoeba
Chlorhexidine
Hydrogen Peroxide
Cysts
polyaminopropylbiguanide
polyquaternium 1

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Silvany, R. E., Dougherty, J. M., & McCulley, J. P. (1991). Effect of contact lens preservatives on Acanthamoeba. Ophthalmology, 98(6), 854-857.

Effect of contact lens preservatives on Acanthamoeba. / Silvany, R. E.; Dougherty, J. M.; McCulley, James P.

In: Ophthalmology, Vol. 98, No. 6, 1991, p. 854-857.

Research output: Contribution to journalArticle

Silvany, RE, Dougherty, JM & McCulley, JP 1991, 'Effect of contact lens preservatives on Acanthamoeba', Ophthalmology, vol. 98, no. 6, pp. 854-857.
Silvany, R. E. ; Dougherty, J. M. ; McCulley, James P. / Effect of contact lens preservatives on Acanthamoeba. In: Ophthalmology. 1991 ; Vol. 98, No. 6. pp. 854-857.
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