TY - JOUR
T1 - Effect of cryopreservation on IL-4, IFNγ and IL-6 production of porcine peripheral blood lymphocytes
AU - Li, Xiujin
AU - Zhong, Zhenyu
AU - Liang, Shuang
AU - Wang, Xingxing
AU - Zhong, Fei
N1 - Funding Information:
Funded by the National Natural Science Foundation of China (Grant No. 30771586 ) and the Hebei Province Natural Science Foundation ( C2008000244 ).
PY - 2009/12
Y1 - 2009/12
N2 - Cryopreservation of animal or human peripheral blood mononuclear cells (PBMC) is a commonly used technique. Effects of cryopreservation on functional capacity, especially the cytokine production of human PBMCs, have been extensively defined. However, certain animals, such as livestock, are a shortage of these information. Here we investigated the effects of cryopreservation on cytokine (IL-4, IFNγ and IL-6) production of porcine PBMC. The porcine PBMCs were cryopreserved at -196 °C for a variety time periods for 2, 5, 25 and 50 days. Viability and cytokine production of the porcine PBMCs were measured before and after cryopreservation. The results showed that about 90% cell recovery rate was obtained at each storage time, indicating that about 10% loss of PBMCs in this short-term cryopreservation was due to the freezing process rather than the duration of cryopreservation. The fresh or frozen resting porcine PBMCs produced little cytokines in the absence of stimulation. However, three cytokines were apparently increased after PMA stimulation in both fresh and frozen porcine PBMCs. The sensitivity of frozen cells to PMA simulation for IFNγ and IL-6 production was different from that of the fresh ones. IFNγ production from the frozen PBMCs was significantly higher than that from the fresh ones (P < 0.01). In contrast, IL-6 level from the frozen sample was significantly lower than that from the fresh one (P < 0.05). Those results indicate that cryopreservation can increase the sensitivity of porcine PBMCs stimulated by PMA for IFNγ production but not for IL-6 production. There was no significant difference of IL-4 production between fresh and frozen cells either stimulated (P > 0.05) or un-stimulated (P > 0.05).
AB - Cryopreservation of animal or human peripheral blood mononuclear cells (PBMC) is a commonly used technique. Effects of cryopreservation on functional capacity, especially the cytokine production of human PBMCs, have been extensively defined. However, certain animals, such as livestock, are a shortage of these information. Here we investigated the effects of cryopreservation on cytokine (IL-4, IFNγ and IL-6) production of porcine PBMC. The porcine PBMCs were cryopreserved at -196 °C for a variety time periods for 2, 5, 25 and 50 days. Viability and cytokine production of the porcine PBMCs were measured before and after cryopreservation. The results showed that about 90% cell recovery rate was obtained at each storage time, indicating that about 10% loss of PBMCs in this short-term cryopreservation was due to the freezing process rather than the duration of cryopreservation. The fresh or frozen resting porcine PBMCs produced little cytokines in the absence of stimulation. However, three cytokines were apparently increased after PMA stimulation in both fresh and frozen porcine PBMCs. The sensitivity of frozen cells to PMA simulation for IFNγ and IL-6 production was different from that of the fresh ones. IFNγ production from the frozen PBMCs was significantly higher than that from the fresh ones (P < 0.01). In contrast, IL-6 level from the frozen sample was significantly lower than that from the fresh one (P < 0.05). Those results indicate that cryopreservation can increase the sensitivity of porcine PBMCs stimulated by PMA for IFNγ production but not for IL-6 production. There was no significant difference of IL-4 production between fresh and frozen cells either stimulated (P > 0.05) or un-stimulated (P > 0.05).
KW - Cryopreservation
KW - Cytokine productions
KW - Peripheral blood mononuclear cells
KW - Porcine
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U2 - 10.1016/j.cryobiol.2009.09.004
DO - 10.1016/j.cryobiol.2009.09.004
M3 - Article
C2 - 19766617
AN - SCOPUS:70649103856
SN - 0011-2240
VL - 59
SP - 322
EP - 326
JO - Cryobiology
JF - Cryobiology
IS - 3
ER -