Effect of cytoarchitecture on the transport and localization of protein synthetic machinery

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The emerging picture of cytoarchitecture imposes constraints on the transport and localization of several components of the protein synthetic machinery. The range in which 'free' polysomes can diffuse through the cytoplasm may be restricted to about 50 nm due to obstruction by cytoskeletal barriers. Individual ribosomes and large transcripts will diffuse at least 4-10 times slower in cytoplasm than in dilute aqueous solution and may be sterically excluded from some cytoplasmic domains. The transport of these components from the nucleus to the cell periphery may be restricted to microtubule-containing channels that traverse the excluding domains. In the peripheral cytoplasm, mitochondria, endoplasmic reticulum, and other membrane-bound organelles are found only in nonexcluding channels, while actin, nonmuscle filamin (ABP280), and fodrin are concentrated in excluding domains. This suggests that the cytoplasmic volume may be functionally compartmentalized by local differentiations of cytoarchitecture. Excluding compartments may play a structural role, while nonexcluding compartments are the site of vesicle traffic and protein synthesis.

Original languageEnglish (US)
Pages (from-to)140-147
Number of pages8
JournalJournal of Cellular Biochemistry
Volume52
Issue number2
StatePublished - 1993

Fingerprint

Machinery
Carrier Proteins
Cytoplasm
Filamins
Mitochondria
Actins
Proteins
Polyribosomes
Cell Nucleus
Membranes
Ribosomes
Microtubules
Endoplasmic Reticulum
Organelles
fodrin

Keywords

  • cytoplasm
  • excluding compartments
  • FRAP data
  • polysomes
  • tracer particles

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Effect of cytoarchitecture on the transport and localization of protein synthetic machinery. / Luby-Phelps, K.

In: Journal of Cellular Biochemistry, Vol. 52, No. 2, 1993, p. 140-147.

Research output: Contribution to journalArticle

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