Effect of Differing Parameters on Irreversible Electroporation in a Porcine Model

Noah E. Canvasser, Aaron H. Lay, Ersin Koseoglu, Nicholas Kavoussi, Igor Sorokin, Jeffrey Gahan, Elena A Lucas, Jeffrey A Cadeddu

Research output: Contribution to journalArticle

Abstract

Introduction and Objective: Irreversible electroporation (IRE) is a new ablative technology to treat small renal masses. We evaluated differed ablation settings on lesion size and temperature changes in a porcine model. Materials and Methods: After Institutional Animal Care and Use Committee approval, 36 laparoscopy-guided and 16 open ablations were performed on 13 domestic female pigs. Ablation parameters studied were voltage (1000 V/cm, 1500 V/cm, or 2000 V/cm), probe exposure (1.0 or 1.5 cm), and lesion size over time (survival) (0-, 7-, or 14 day). Temperature changes were monitored during open ablations with differed settings. Gross lesion size was measured, and histologic analysis with hematoxylin and eosin and nicotinamide adenine dinucleotide staining was performed. Results: The 1000 V/cm ablations had no gross or histologic lesions. A factorial analysis of variance demonstrated that day (p = 0.56), exposure (p = 0.33), and voltage (p = 0.06) did not demonstrate statistical significance for affecting lesion size. For 1.0 cm probe exposure, 2000 V/cm did more closely approximate expected lesion size (p = 0.02) compared with 1500 V/cm. While significance was not seen for 1.5 cm probe exposure, 2000 V/cm often exceeded expected lesion volume. Only 1 of 4 temperature sensors, located adjacent to one of the IRE probes, noted a significant increase with increased voltage. However, all maximum temperatures remained less than 70°C. Conclusions: Variation in lesion volume was seen with different ablation settings in this porcine model. Maximal energy and probe exposure settings should be utilized to ensure full coverage of target volume/mass, potentially without concern for thermal injury to renal collecting system or nearby structures.

Original languageEnglish (US)
Pages (from-to)338-343
Number of pages6
JournalJournal of Endourology
Volume32
Issue number4
DOIs
StatePublished - Apr 1 2018

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Electroporation
Swine
Temperature
Animal Care Committees
Kidney
Sus scrofa
Hematoxylin
Eosine Yellowish-(YS)
NAD
Laparoscopy
Analysis of Variance
Hot Temperature
Staining and Labeling
Technology
Wounds and Injuries

Keywords

  • electroporation, kidney neoplasm, histology

ASJC Scopus subject areas

  • Urology

Cite this

Effect of Differing Parameters on Irreversible Electroporation in a Porcine Model. / Canvasser, Noah E.; Lay, Aaron H.; Koseoglu, Ersin; Kavoussi, Nicholas; Sorokin, Igor; Gahan, Jeffrey; Lucas, Elena A; Cadeddu, Jeffrey A.

In: Journal of Endourology, Vol. 32, No. 4, 01.04.2018, p. 338-343.

Research output: Contribution to journalArticle

Canvasser, Noah E. ; Lay, Aaron H. ; Koseoglu, Ersin ; Kavoussi, Nicholas ; Sorokin, Igor ; Gahan, Jeffrey ; Lucas, Elena A ; Cadeddu, Jeffrey A. / Effect of Differing Parameters on Irreversible Electroporation in a Porcine Model. In: Journal of Endourology. 2018 ; Vol. 32, No. 4. pp. 338-343.
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