Effect of dopamine system activation on substantia nigra pars reticulata output neurons

Variable single-unit responses in normal rats and inhibition in 6-hydroxydopamine-lesioned rats

B. L. Waszczak, E. K. Lee, C. A. Tamminga, J. R. Walters

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Abstract

Previous single-unit recording studies have revealed that randomly selected pars reticulata neurons respond in a highly variable and complex fashion to intravenous administration of the dopamine agonist, apomorphine. The current studies were undertaken to assess whether the variable pattern of responses of reticulata neurons to intravenous apomorphine might be altered by the presence of striatal dopaminergic supersensitivity. Extracellular, single-unit recording studies were conducted in anesthetized, paralyzed rats. Pars reticulata neurons were identified by antidromic activation from either the ventromedial nucleus of the thalamus or superior colliculus. Neurons of both subpopulations exhibited similar, highly variable changes in firing rate during the 10-min period immediately following intravenous injection of 320 μg/kg of apomorphine, a dose of the drug considered sufficient to stimulate striatal postsynaptic dopamine receptors. These responses, which were not qualitatively different from those previously observed among reticulata cells not distinguished on the basis of projection site, could be reversed by subsequent administration of dopamine antagonist drugs. IN contrast to the variable responses in normal animals, the same dose of apomorphine caused a rapid and usually total inhibition of pars reticulata cell firing in rats which received 6-hydroxydopamine lesions of the nigrostriatal dopamine pathway 6 to 8 weeks prior to recording experiments. These inhibitions of firing could also be reversed by administration of dopamine antagonists. The results demonstrate that the variable responses of pars reticulata neurons to intravenous apomorphine are not dependent on projection site and, therefore, suggest that dopamine system activation typically results in transmission of complex, nonuniform messages to several, and perhaps all, reticulata output sites. However, the presence of striatal dopaminergic supersensitivity appears to exaggerate inhibitory, dopamine-mediated influences upon pars reticulata output neurons selectively and consistently.

Original languageEnglish (US)
Pages (from-to)2369-2375
Number of pages7
JournalJournal of Neuroscience
Volume4
Issue number9
StatePublished - 1984

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Oxidopamine
Apomorphine
Dopamine
Neurons
Corpus Striatum
Dopamine Antagonists
Dopamine Agents
Superior Colliculi
Dopamine Agonists
Dopamine Receptors
Thalamus
Intravenous Injections
Intravenous Administration
Pars Reticulata
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "Effect of dopamine system activation on substantia nigra pars reticulata output neurons: Variable single-unit responses in normal rats and inhibition in 6-hydroxydopamine-lesioned rats",
abstract = "Previous single-unit recording studies have revealed that randomly selected pars reticulata neurons respond in a highly variable and complex fashion to intravenous administration of the dopamine agonist, apomorphine. The current studies were undertaken to assess whether the variable pattern of responses of reticulata neurons to intravenous apomorphine might be altered by the presence of striatal dopaminergic supersensitivity. Extracellular, single-unit recording studies were conducted in anesthetized, paralyzed rats. Pars reticulata neurons were identified by antidromic activation from either the ventromedial nucleus of the thalamus or superior colliculus. Neurons of both subpopulations exhibited similar, highly variable changes in firing rate during the 10-min period immediately following intravenous injection of 320 μg/kg of apomorphine, a dose of the drug considered sufficient to stimulate striatal postsynaptic dopamine receptors. These responses, which were not qualitatively different from those previously observed among reticulata cells not distinguished on the basis of projection site, could be reversed by subsequent administration of dopamine antagonist drugs. IN contrast to the variable responses in normal animals, the same dose of apomorphine caused a rapid and usually total inhibition of pars reticulata cell firing in rats which received 6-hydroxydopamine lesions of the nigrostriatal dopamine pathway 6 to 8 weeks prior to recording experiments. These inhibitions of firing could also be reversed by administration of dopamine antagonists. The results demonstrate that the variable responses of pars reticulata neurons to intravenous apomorphine are not dependent on projection site and, therefore, suggest that dopamine system activation typically results in transmission of complex, nonuniform messages to several, and perhaps all, reticulata output sites. However, the presence of striatal dopaminergic supersensitivity appears to exaggerate inhibitory, dopamine-mediated influences upon pars reticulata output neurons selectively and consistently.",
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T1 - Effect of dopamine system activation on substantia nigra pars reticulata output neurons

T2 - Variable single-unit responses in normal rats and inhibition in 6-hydroxydopamine-lesioned rats

AU - Waszczak, B. L.

AU - Lee, E. K.

AU - Tamminga, C. A.

AU - Walters, J. R.

PY - 1984

Y1 - 1984

N2 - Previous single-unit recording studies have revealed that randomly selected pars reticulata neurons respond in a highly variable and complex fashion to intravenous administration of the dopamine agonist, apomorphine. The current studies were undertaken to assess whether the variable pattern of responses of reticulata neurons to intravenous apomorphine might be altered by the presence of striatal dopaminergic supersensitivity. Extracellular, single-unit recording studies were conducted in anesthetized, paralyzed rats. Pars reticulata neurons were identified by antidromic activation from either the ventromedial nucleus of the thalamus or superior colliculus. Neurons of both subpopulations exhibited similar, highly variable changes in firing rate during the 10-min period immediately following intravenous injection of 320 μg/kg of apomorphine, a dose of the drug considered sufficient to stimulate striatal postsynaptic dopamine receptors. These responses, which were not qualitatively different from those previously observed among reticulata cells not distinguished on the basis of projection site, could be reversed by subsequent administration of dopamine antagonist drugs. IN contrast to the variable responses in normal animals, the same dose of apomorphine caused a rapid and usually total inhibition of pars reticulata cell firing in rats which received 6-hydroxydopamine lesions of the nigrostriatal dopamine pathway 6 to 8 weeks prior to recording experiments. These inhibitions of firing could also be reversed by administration of dopamine antagonists. The results demonstrate that the variable responses of pars reticulata neurons to intravenous apomorphine are not dependent on projection site and, therefore, suggest that dopamine system activation typically results in transmission of complex, nonuniform messages to several, and perhaps all, reticulata output sites. However, the presence of striatal dopaminergic supersensitivity appears to exaggerate inhibitory, dopamine-mediated influences upon pars reticulata output neurons selectively and consistently.

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