Effect of epoxyeicosatrienoic acids on growth hormone release from somatotrophs

G. D. Snyder, P. Yadagiri, J R Falck

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Growth hormone secretion was stimulated in vitro by products of arachidonic acid epoxygenase, the epoxyeicosatrienoic acids. 5,6-Epoxyeicosatrienoic and 14,15-epoxyeicosatrienoic acid stimulated growth hormone release from an enriched population of somatotrophs (~85%) by twofold. Inhibition of arachidonic acid metabolism by indomethacin did not affect growth hormone-releasing hormone stimulation of growth hormone release. In contast, pretreatment of somatotrophs with an 11,12-isonitrile analogue of arachidonic acid that inhibits arachidonic acid epoxygenase, resulted in a 20-25% inhibition of growth hormone-releasing hormone-stimulated growth hormone release. 14,15-Epoxyeicosatrienoic acid stimulated a concentration-dependent increase (twofold) in the cytoplasmic concentration of adenosine 3',5'-cyclic monophosphate (cAMP) in the somatotrophs. 14,15-Epoxyeicosatrienoic acid also rapidly increased the intracellular free calcium concentration in somatotrophs from resting levels (~80 nM) to > 250 nM. Growth hormone-leasing hormone increased the free intracellular calcium to 160-180 nM. Preincubation of somatotrophs with somatostatin inhibited growth hormone-releasing hormone-stimulated growth hormone secretion, cAMP accumulation, and 14,15-epoxyeicosatrienoic acid stimulated cAMP accumulation. These data are suggestive that the epoxyeicosatrienoic acids may have a role in the secretion of growth hormone.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume256
Issue number2
StatePublished - 1989

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Somatotrophs
Growth Hormone
Acids
Growth Hormone-Releasing Hormone
Cyclic AMP
Arachidonic Acid
Calcium
Enzyme inhibition
Somatostatin
Metabolism
Indomethacin
Byproducts
Hormones
14,15-epoxy-5,8,11-eicosatrienoic acid

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology

Cite this

Effect of epoxyeicosatrienoic acids on growth hormone release from somatotrophs. / Snyder, G. D.; Yadagiri, P.; Falck, J R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 256, No. 2, 1989.

Research output: Contribution to journalArticle

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