Effect of feeding psyllium and cholestyramine in combination on low density lipoprotein metabolism and fecal bile acid excretion in hamsters with dietary-induced hypercholesterolemia

Stephen D. Turley, Bruce P. Daggy, John M. Dietschy

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We wished to determine the effectiveness of submaximal doses of cholestyramine and psyllium given in combination in reversing dietary- induced hypercholesterolemia in Golden Syrian hamsters, and to investigate the mechanism or mechanisms of action through which these agents together decrease plasma low density lipoprotein cholesterol (LDL-C) levels in this model. For 30 days, male hamsters were fed a cholesterol-rich cereal-based diet containing either a submaximal dose of cholestyramine (1% wt/wt) alone or in combination with psyllium (either 2 or 4%), or a high dose of cholestyramine (3%) alone. Although the greatest cholesterol-reducing action was achieved with 3% resin alone, in the animals fed one third as much cholestyramine combined with psyllium (4%) LDL-C production decreased from 288 ± 15 to 187 ± 17 μg/h per 100 g body weight, the suppression of LDL- receptor activity was almost fully reversed, plasma LDL-C levels were reduced from 90 ± 8 to 41 ± 5 mg/dl, and hepatic cholesterol content decreased from 17.1 ± 1.9 to 2.4 ± 0.1 mg/g. In the group that received 1% resin alone, the plasma LDL-C and hepatic cholesterol levels were 60 ± 3 mg/dl and 7.2 ± 0.6 mg/g, respectively. As compared with animals that received 1% resin alone, those fed both agents manifested higher rates of fecal bile acid excretion and lower levels of intestinal cholesterol absorption. A significant cholesterol-lowering benefit can he derived from using these nonsystemic agents in combination at lower, more tolerable doses.

Original languageEnglish (US)
Pages (from-to)71-79
Number of pages9
JournalJournal of Cardiovascular Pharmacology
Volume27
Issue number1
DOIs
StatePublished - 1996

Fingerprint

Psyllium
Cholestyramine Resin
Hypercholesterolemia
Bile Acids and Salts
LDL Lipoproteins
Cricetinae
Cholesterol
LDL Cholesterol
LDL Receptors
Liver
Mesocricetus
Intestinal Absorption
Body Weight
Diet

Keywords

  • Bile acids
  • Cholestyramine
  • Hamsters
  • Low density lipoprotein-cholesterol metabolism
  • Psyllium
  • Soluble fiber

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

@article{993a1a20eb74466d8226f50586d216bd,
title = "Effect of feeding psyllium and cholestyramine in combination on low density lipoprotein metabolism and fecal bile acid excretion in hamsters with dietary-induced hypercholesterolemia",
abstract = "We wished to determine the effectiveness of submaximal doses of cholestyramine and psyllium given in combination in reversing dietary- induced hypercholesterolemia in Golden Syrian hamsters, and to investigate the mechanism or mechanisms of action through which these agents together decrease plasma low density lipoprotein cholesterol (LDL-C) levels in this model. For 30 days, male hamsters were fed a cholesterol-rich cereal-based diet containing either a submaximal dose of cholestyramine (1{\%} wt/wt) alone or in combination with psyllium (either 2 or 4{\%}), or a high dose of cholestyramine (3{\%}) alone. Although the greatest cholesterol-reducing action was achieved with 3{\%} resin alone, in the animals fed one third as much cholestyramine combined with psyllium (4{\%}) LDL-C production decreased from 288 ± 15 to 187 ± 17 μg/h per 100 g body weight, the suppression of LDL- receptor activity was almost fully reversed, plasma LDL-C levels were reduced from 90 ± 8 to 41 ± 5 mg/dl, and hepatic cholesterol content decreased from 17.1 ± 1.9 to 2.4 ± 0.1 mg/g. In the group that received 1{\%} resin alone, the plasma LDL-C and hepatic cholesterol levels were 60 ± 3 mg/dl and 7.2 ± 0.6 mg/g, respectively. As compared with animals that received 1{\%} resin alone, those fed both agents manifested higher rates of fecal bile acid excretion and lower levels of intestinal cholesterol absorption. A significant cholesterol-lowering benefit can he derived from using these nonsystemic agents in combination at lower, more tolerable doses.",
keywords = "Bile acids, Cholestyramine, Hamsters, Low density lipoprotein-cholesterol metabolism, Psyllium, Soluble fiber",
author = "Turley, {Stephen D.} and Daggy, {Bruce P.} and Dietschy, {John M.}",
year = "1996",
doi = "10.1097/00005344-199601000-00012",
language = "English (US)",
volume = "27",
pages = "71--79",
journal = "Journal of Cardiovascular Pharmacology",
issn = "0160-2446",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Effect of feeding psyllium and cholestyramine in combination on low density lipoprotein metabolism and fecal bile acid excretion in hamsters with dietary-induced hypercholesterolemia

AU - Turley, Stephen D.

AU - Daggy, Bruce P.

AU - Dietschy, John M.

PY - 1996

Y1 - 1996

N2 - We wished to determine the effectiveness of submaximal doses of cholestyramine and psyllium given in combination in reversing dietary- induced hypercholesterolemia in Golden Syrian hamsters, and to investigate the mechanism or mechanisms of action through which these agents together decrease plasma low density lipoprotein cholesterol (LDL-C) levels in this model. For 30 days, male hamsters were fed a cholesterol-rich cereal-based diet containing either a submaximal dose of cholestyramine (1% wt/wt) alone or in combination with psyllium (either 2 or 4%), or a high dose of cholestyramine (3%) alone. Although the greatest cholesterol-reducing action was achieved with 3% resin alone, in the animals fed one third as much cholestyramine combined with psyllium (4%) LDL-C production decreased from 288 ± 15 to 187 ± 17 μg/h per 100 g body weight, the suppression of LDL- receptor activity was almost fully reversed, plasma LDL-C levels were reduced from 90 ± 8 to 41 ± 5 mg/dl, and hepatic cholesterol content decreased from 17.1 ± 1.9 to 2.4 ± 0.1 mg/g. In the group that received 1% resin alone, the plasma LDL-C and hepatic cholesterol levels were 60 ± 3 mg/dl and 7.2 ± 0.6 mg/g, respectively. As compared with animals that received 1% resin alone, those fed both agents manifested higher rates of fecal bile acid excretion and lower levels of intestinal cholesterol absorption. A significant cholesterol-lowering benefit can he derived from using these nonsystemic agents in combination at lower, more tolerable doses.

AB - We wished to determine the effectiveness of submaximal doses of cholestyramine and psyllium given in combination in reversing dietary- induced hypercholesterolemia in Golden Syrian hamsters, and to investigate the mechanism or mechanisms of action through which these agents together decrease plasma low density lipoprotein cholesterol (LDL-C) levels in this model. For 30 days, male hamsters were fed a cholesterol-rich cereal-based diet containing either a submaximal dose of cholestyramine (1% wt/wt) alone or in combination with psyllium (either 2 or 4%), or a high dose of cholestyramine (3%) alone. Although the greatest cholesterol-reducing action was achieved with 3% resin alone, in the animals fed one third as much cholestyramine combined with psyllium (4%) LDL-C production decreased from 288 ± 15 to 187 ± 17 μg/h per 100 g body weight, the suppression of LDL- receptor activity was almost fully reversed, plasma LDL-C levels were reduced from 90 ± 8 to 41 ± 5 mg/dl, and hepatic cholesterol content decreased from 17.1 ± 1.9 to 2.4 ± 0.1 mg/g. In the group that received 1% resin alone, the plasma LDL-C and hepatic cholesterol levels were 60 ± 3 mg/dl and 7.2 ± 0.6 mg/g, respectively. As compared with animals that received 1% resin alone, those fed both agents manifested higher rates of fecal bile acid excretion and lower levels of intestinal cholesterol absorption. A significant cholesterol-lowering benefit can he derived from using these nonsystemic agents in combination at lower, more tolerable doses.

KW - Bile acids

KW - Cholestyramine

KW - Hamsters

KW - Low density lipoprotein-cholesterol metabolism

KW - Psyllium

KW - Soluble fiber

UR - http://www.scopus.com/inward/record.url?scp=0030044508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030044508&partnerID=8YFLogxK

U2 - 10.1097/00005344-199601000-00012

DO - 10.1097/00005344-199601000-00012

M3 - Article

C2 - 8656662

AN - SCOPUS:0030044508

VL - 27

SP - 71

EP - 79

JO - Journal of Cardiovascular Pharmacology

JF - Journal of Cardiovascular Pharmacology

SN - 0160-2446

IS - 1

ER -