Effect of heat shock response on the cleavage of nucleolin induced by oxidative stress

Kang Kai Wang, Lei Jiang, Yu Xin Yi, Ke Liu, E. Shun-Mei, Dao Lin Tang, Jian She Wang, Yong Zhong Shi, Qiu Peng Wang, Xian Zhong Xiao

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Objective: To observe the cleavage of nucleolin (C23) during apoptosis induced by oxidative stress and to clarify the effect of heat shock response (HSR) on the cleavage of nucleolin and its possible molecular mechanism. Methods: We added 0. 5 mmol/L peroxide hydrogen (H 2O 2) into cultured cells to mimic oxidative stress. Apoptosis and cleavage of C23 were detected using caspase-3 colorimetric assay and Western blotting respectively. HSR was performed to observe the effect of HSR on cleavage of C23 induced by oxidative stress, and overexpressions of HSP70 and HSP25 were detected by Western blotting. Results: Activity of caspase-3 increased significantly after 2 hours of 0. 5 mmol/L H 2O 2treatment, and reached the peak after 12 hours. The cleavage of C23 appeared 30 minutes to 1 hour after the treatment of H 2O 2 as indicated by a cleaved fragmentation of 80 kD, which was significantly inhibited by HSR. Moreover, HSR could induce HSP70 and HSP25 overexpressions. Conclusion: Oxidative stress can induce the activation of caspase -3, cleavage of C23, and apoptosis. HSR can significantly inhibit the cleavage of C23 induced by oxidative stress, which is related to the overexpressions of HSP70, HSP25, and other stress proteins.

Original languageEnglish (US)
Pages (from-to)504-508
Number of pages5
JournalJournal of Central South University (Medical Sciences)
Volume29
Issue number5
StatePublished - Oct 28 2004
Externally publishedYes

Keywords

  • Heat shock response
  • Nucleolin
  • Oxidative stress
  • Peroxide hydrogen

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Wang, K. K., Jiang, L., Yi, Y. X., Liu, K., Shun-Mei, E., Tang, D. L., Wang, J. S., Shi, Y. Z., Wang, Q. P., & Xiao, X. Z. (2004). Effect of heat shock response on the cleavage of nucleolin induced by oxidative stress. Journal of Central South University (Medical Sciences), 29(5), 504-508.