Effect of human cytomegalovirus on expression of MHC class I-related chains A

Yizhou Zou, Wade Bresnahan, R. Travis Taylor, Peter Stastny

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The MHC-encoded MHC class I-related chains A (MICA) glycoproteins are known to enhance the functions of NK and T cells by ligating the stimulating receptor NKG2D and appear to play an important role in host defense. Human CMV (HCMV) evades the immune response in many different ways, but has not previously been found to down-regulate MICA. We have found that a common form of MICA, which has a nucleotide insertion in exon 5 corresponding to the transmembrane region and no cytoplasmic tail, was increased on the surface of fibroblasts HFS-13 compared with the mock-infected sample of the same cells that had been cultured to confluence. However, an astrocytoma cell line, U373, which has a full-length variant of MICA, showed that the expression of MICA was decreased after HCMV infection. Retroviral transduction of different MICA alleles into fibroblasts HFF-D, which express no MICA of their own, established that full-length MICA was down-regulated by HCMV, and the truncated form was not. Fibroblasts with decreased MICA due to HCMV infection were found to be protected from NK cell killing, whereas in the presence of the truncated form of MICA, the virus-infected cells were destroyed. Thus, the truncated form of MICA, which is the most common, has a mutation that allows it to persist on the surface and hinder efforts of the virus to evade the immune response.

Original languageEnglish (US)
Pages (from-to)3098-3104
Number of pages7
JournalJournal of Immunology
Volume174
Issue number5
StatePublished - Mar 1 2005

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Cytomegalovirus
Fibroblasts
Natural Killer Cells
MHC class I-related chain A
NK Cell Lectin-Like Receptor Subfamily K
Viruses
Astrocytoma
Infection
Tail
Exons
Glycoproteins
Down-Regulation
Nucleotides
Alleles
T-Lymphocytes
Cell Line
Mutation

ASJC Scopus subject areas

  • Immunology

Cite this

Zou, Y., Bresnahan, W., Taylor, R. T., & Stastny, P. (2005). Effect of human cytomegalovirus on expression of MHC class I-related chains A. Journal of Immunology, 174(5), 3098-3104.

Effect of human cytomegalovirus on expression of MHC class I-related chains A. / Zou, Yizhou; Bresnahan, Wade; Taylor, R. Travis; Stastny, Peter.

In: Journal of Immunology, Vol. 174, No. 5, 01.03.2005, p. 3098-3104.

Research output: Contribution to journalArticle

Zou, Y, Bresnahan, W, Taylor, RT & Stastny, P 2005, 'Effect of human cytomegalovirus on expression of MHC class I-related chains A', Journal of Immunology, vol. 174, no. 5, pp. 3098-3104.
Zou Y, Bresnahan W, Taylor RT, Stastny P. Effect of human cytomegalovirus on expression of MHC class I-related chains A. Journal of Immunology. 2005 Mar 1;174(5):3098-3104.
Zou, Yizhou ; Bresnahan, Wade ; Taylor, R. Travis ; Stastny, Peter. / Effect of human cytomegalovirus on expression of MHC class I-related chains A. In: Journal of Immunology. 2005 ; Vol. 174, No. 5. pp. 3098-3104.
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