Effect of in ovo immobilization on development of chick hind-limb articular cartilage: An evaluation using micro-MRI measurement of delayed gadolinium uptake

Chigusa Sawamura, Masaya Takahashi, Kathryn J. McCarthy, Zhenxin Shen, Naomi Fukai, Edward K. Rodriguez, Brian D. Snyder

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To examine the effect of immobilization on the development of articular cartilage, we assessed glycosaminoglycan (GAG) content in the chick articular surface by delayed gadolinium-enhanced MRI of cartilage (dGEMRIC). Chick embryos were paralyzed by decamethonium bromide (DMB) from day 10 to either day 13 or day 16. The GAG content of the chick knee was compared with that of nonparalyzed chick embryos. Histologic analysis was unable to quantify GAG content; however, dGEMRIC demonstrated that GAG content was higher in the femoral condyles of the nonparalyzed embryos on day 13, and on day 16 the GAG content was lower in both the femoral condyles and the tibial plateaus of the nonparalyzed embryos. These results suggest that paralysis delays embryonic hind-limb development. Osteoblastic activity at the cartilage canal, as demonstrated by staining for alkaline phosphatase (ALP), was present only in the nonparalyzed chick embryos on day 16. The GAG content of the cartilage decreased when the cartilage canals began to form on day 16. The effect of immobilization on hindlimb development was indicated by the differences in the GAG content of the cartilage anlage measured by dGEMRIC in the developing knee joint of paralyzed and nonparalyzed embryonic chicks.

Original languageEnglish (US)
Pages (from-to)1235-1241
Number of pages7
JournalMagnetic Resonance in Medicine
Volume56
Issue number6
DOIs
StatePublished - Dec 2006

Fingerprint

Gadolinium
Articular Cartilage
Glycosaminoglycans
Immobilization
Cartilage
Extremities
Chick Embryo
Thigh
Embryonic Structures
Hindlimb Suspension
Bone and Bones
Knee Joint
Paralysis
Alkaline Phosphatase
Knee
Joints
Staining and Labeling

Keywords

  • Chick embryo
  • Development
  • dGEMRIC
  • MRI

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Effect of in ovo immobilization on development of chick hind-limb articular cartilage : An evaluation using micro-MRI measurement of delayed gadolinium uptake. / Sawamura, Chigusa; Takahashi, Masaya; McCarthy, Kathryn J.; Shen, Zhenxin; Fukai, Naomi; Rodriguez, Edward K.; Snyder, Brian D.

In: Magnetic Resonance in Medicine, Vol. 56, No. 6, 12.2006, p. 1235-1241.

Research output: Contribution to journalArticle

Sawamura, Chigusa ; Takahashi, Masaya ; McCarthy, Kathryn J. ; Shen, Zhenxin ; Fukai, Naomi ; Rodriguez, Edward K. ; Snyder, Brian D. / Effect of in ovo immobilization on development of chick hind-limb articular cartilage : An evaluation using micro-MRI measurement of delayed gadolinium uptake. In: Magnetic Resonance in Medicine. 2006 ; Vol. 56, No. 6. pp. 1235-1241.
@article{48ab958c3785480bbd2ad1a09296756d,
title = "Effect of in ovo immobilization on development of chick hind-limb articular cartilage: An evaluation using micro-MRI measurement of delayed gadolinium uptake",
abstract = "To examine the effect of immobilization on the development of articular cartilage, we assessed glycosaminoglycan (GAG) content in the chick articular surface by delayed gadolinium-enhanced MRI of cartilage (dGEMRIC). Chick embryos were paralyzed by decamethonium bromide (DMB) from day 10 to either day 13 or day 16. The GAG content of the chick knee was compared with that of nonparalyzed chick embryos. Histologic analysis was unable to quantify GAG content; however, dGEMRIC demonstrated that GAG content was higher in the femoral condyles of the nonparalyzed embryos on day 13, and on day 16 the GAG content was lower in both the femoral condyles and the tibial plateaus of the nonparalyzed embryos. These results suggest that paralysis delays embryonic hind-limb development. Osteoblastic activity at the cartilage canal, as demonstrated by staining for alkaline phosphatase (ALP), was present only in the nonparalyzed chick embryos on day 16. The GAG content of the cartilage decreased when the cartilage canals began to form on day 16. The effect of immobilization on hindlimb development was indicated by the differences in the GAG content of the cartilage anlage measured by dGEMRIC in the developing knee joint of paralyzed and nonparalyzed embryonic chicks.",
keywords = "Chick embryo, Development, dGEMRIC, MRI",
author = "Chigusa Sawamura and Masaya Takahashi and McCarthy, {Kathryn J.} and Zhenxin Shen and Naomi Fukai and Rodriguez, {Edward K.} and Snyder, {Brian D.}",
year = "2006",
month = "12",
doi = "10.1002/mrm.21021",
language = "English (US)",
volume = "56",
pages = "1235--1241",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Effect of in ovo immobilization on development of chick hind-limb articular cartilage

T2 - An evaluation using micro-MRI measurement of delayed gadolinium uptake

AU - Sawamura, Chigusa

AU - Takahashi, Masaya

AU - McCarthy, Kathryn J.

AU - Shen, Zhenxin

AU - Fukai, Naomi

AU - Rodriguez, Edward K.

AU - Snyder, Brian D.

PY - 2006/12

Y1 - 2006/12

N2 - To examine the effect of immobilization on the development of articular cartilage, we assessed glycosaminoglycan (GAG) content in the chick articular surface by delayed gadolinium-enhanced MRI of cartilage (dGEMRIC). Chick embryos were paralyzed by decamethonium bromide (DMB) from day 10 to either day 13 or day 16. The GAG content of the chick knee was compared with that of nonparalyzed chick embryos. Histologic analysis was unable to quantify GAG content; however, dGEMRIC demonstrated that GAG content was higher in the femoral condyles of the nonparalyzed embryos on day 13, and on day 16 the GAG content was lower in both the femoral condyles and the tibial plateaus of the nonparalyzed embryos. These results suggest that paralysis delays embryonic hind-limb development. Osteoblastic activity at the cartilage canal, as demonstrated by staining for alkaline phosphatase (ALP), was present only in the nonparalyzed chick embryos on day 16. The GAG content of the cartilage decreased when the cartilage canals began to form on day 16. The effect of immobilization on hindlimb development was indicated by the differences in the GAG content of the cartilage anlage measured by dGEMRIC in the developing knee joint of paralyzed and nonparalyzed embryonic chicks.

AB - To examine the effect of immobilization on the development of articular cartilage, we assessed glycosaminoglycan (GAG) content in the chick articular surface by delayed gadolinium-enhanced MRI of cartilage (dGEMRIC). Chick embryos were paralyzed by decamethonium bromide (DMB) from day 10 to either day 13 or day 16. The GAG content of the chick knee was compared with that of nonparalyzed chick embryos. Histologic analysis was unable to quantify GAG content; however, dGEMRIC demonstrated that GAG content was higher in the femoral condyles of the nonparalyzed embryos on day 13, and on day 16 the GAG content was lower in both the femoral condyles and the tibial plateaus of the nonparalyzed embryos. These results suggest that paralysis delays embryonic hind-limb development. Osteoblastic activity at the cartilage canal, as demonstrated by staining for alkaline phosphatase (ALP), was present only in the nonparalyzed chick embryos on day 16. The GAG content of the cartilage decreased when the cartilage canals began to form on day 16. The effect of immobilization on hindlimb development was indicated by the differences in the GAG content of the cartilage anlage measured by dGEMRIC in the developing knee joint of paralyzed and nonparalyzed embryonic chicks.

KW - Chick embryo

KW - Development

KW - dGEMRIC

KW - MRI

UR - http://www.scopus.com/inward/record.url?scp=33845264172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845264172&partnerID=8YFLogxK

U2 - 10.1002/mrm.21021

DO - 10.1002/mrm.21021

M3 - Article

C2 - 17089363

AN - SCOPUS:33845264172

VL - 56

SP - 1235

EP - 1241

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

SN - 0740-3194

IS - 6

ER -