Effect of Ischemia on NMR Detection of Phosphorylated Metabolites in the Intact Rat Heart

F. Mark Jeffrey, Charles J. Storey, Ray L. Nunnally, Craig R. Malloy

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Phosphorus NMR spectroscopy is an important technique for the investigation of metabolism in tissues and intact organisms (including man). However, quantitation of the signals from an NMR experiment is difficult because it is not known from which regions of a cell metabolites are detected. It is generally believed that only metabolites free in the cytosol are observed. In this study a comparison of concentration measurements obtained by NMR and after freeze extraction was made in the normoxic and ischemic rat heart. The influence of ischemia was examined because of its potential effect on the level of phosphate metabolites in various compartments. The same fraction of ATP always appears visible to NMR, whereas inorganic phosphate is largely NMR invisible until after a period of ischemia and the phosphomonoesters are only partially observed early in ischemia.

Original languageEnglish (US)
Pages (from-to)5323-5326
Number of pages4
JournalBiochemistry
Volume28
Issue number13
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Effect of Ischemia on NMR Detection of Phosphorylated Metabolites in the Intact Rat Heart'. Together they form a unique fingerprint.

Cite this