Effect of Ischemia on NMR Detection of Phosphorylated Metabolites in the Intact Rat Heart

F. Mark Jeffrey; Charles J. Storey; Ray L. Nunnally; Craig R. Malloy

doi:10.1021/bi00439a003

Effect of Ischemia on NMR Detection of Phosphorylated Metabolites in the Intact Rat Heart

F. Mark Jeffrey, Charles J. Storey, Ray L. Nunnally, Craig R. Malloy

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Abstract

Phosphorus NMR spectroscopy is an important technique for the investigation of metabolism in tissues and intact organisms (including man). However, quantitation of the signals from an NMR experiment is difficult because it is not known from which regions of a cell metabolites are detected. It is generally believed that only metabolites free in the cytosol are observed. In this study a comparison of concentration measurements obtained by NMR and after freeze extraction was made in the normoxic and ischemic rat heart. The influence of ischemia was examined because of its potential effect on the level of phosphate metabolites in various compartments. The same fraction of ATP always appears visible to NMR, whereas inorganic phosphate is largely NMR invisible until after a period of ischemia and the phosphomonoesters are only partially observed early in ischemia.

Original language	English (US)
Pages (from-to)	5323-5326
Number of pages	4
Journal	Biochemistry
Volume	28
Issue number	13
DOIs	https://doi.org/10.1021/bi00439a003
State	Published - 1989

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Biochemistry

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title = "Effect of Ischemia on NMR Detection of Phosphorylated Metabolites in the Intact Rat Heart",

abstract = "Phosphorus NMR spectroscopy is an important technique for the investigation of metabolism in tissues and intact organisms (including man). However, quantitation of the signals from an NMR experiment is difficult because it is not known from which regions of a cell metabolites are detected. It is generally believed that only metabolites free in the cytosol are observed. In this study a comparison of concentration measurements obtained by NMR and after freeze extraction was made in the normoxic and ischemic rat heart. The influence of ischemia was examined because of its potential effect on the level of phosphate metabolites in various compartments. The same fraction of ATP always appears visible to NMR, whereas inorganic phosphate is largely NMR invisible until after a period of ischemia and the phosphomonoesters are only partially observed early in ischemia.",

author = "{Mark Jeffrey}, F. and Storey, {Charles J.} and Nunnally, {Ray L.} and Malloy, {Craig R.}",

year = "1989",

doi = "10.1021/bi00439a003",

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journal = "Biochemistry",

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T1 - Effect of Ischemia on NMR Detection of Phosphorylated Metabolites in the Intact Rat Heart

AU - Mark Jeffrey, F.

AU - Storey, Charles J.

AU - Nunnally, Ray L.

AU - Malloy, Craig R.

PY - 1989

Y1 - 1989

N2 - Phosphorus NMR spectroscopy is an important technique for the investigation of metabolism in tissues and intact organisms (including man). However, quantitation of the signals from an NMR experiment is difficult because it is not known from which regions of a cell metabolites are detected. It is generally believed that only metabolites free in the cytosol are observed. In this study a comparison of concentration measurements obtained by NMR and after freeze extraction was made in the normoxic and ischemic rat heart. The influence of ischemia was examined because of its potential effect on the level of phosphate metabolites in various compartments. The same fraction of ATP always appears visible to NMR, whereas inorganic phosphate is largely NMR invisible until after a period of ischemia and the phosphomonoesters are only partially observed early in ischemia.

AB - Phosphorus NMR spectroscopy is an important technique for the investigation of metabolism in tissues and intact organisms (including man). However, quantitation of the signals from an NMR experiment is difficult because it is not known from which regions of a cell metabolites are detected. It is generally believed that only metabolites free in the cytosol are observed. In this study a comparison of concentration measurements obtained by NMR and after freeze extraction was made in the normoxic and ischemic rat heart. The influence of ischemia was examined because of its potential effect on the level of phosphate metabolites in various compartments. The same fraction of ATP always appears visible to NMR, whereas inorganic phosphate is largely NMR invisible until after a period of ischemia and the phosphomonoesters are only partially observed early in ischemia.

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Effect of Ischemia on NMR Detection of Phosphorylated Metabolites in the Intact Rat Heart

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