Effect of ischemia on NMR detection of phosphorylated metabolites in the intact rat heart

F. Mark Jeffrey, Charles J. Storey, Ray L. Nunnally, Craig R. Malloy

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Phosphorus NMR spectroscopy is an important technique for the investigation of metabolism in tissues and intact organisms (including man). However, quantitation of the signals from an NMR experiment is difficult because it is not known from which regions of a cell metabolites are detected. It is generally believed that only metabolites free in the cytosol are observed. In this study a comparison of concentration measurements obtained by NMR and after freeze extraction was made in the normoxic and ischemic rat heart. The influence of ischemia was examined because of its potential effect on the level of phosphate metabolites in various compartments. The same fraction of ATP always appears visible to NMR, whereas inorganic phosphate is largely NMR invisible until after a period of ischemia and the phosphomonoesters are only partially observed early in ischemia.

Original languageEnglish (US)
Pages (from-to)5323-5326
Number of pages4
JournalBiochemistry
Volume28
Issue number13
StatePublished - 1989

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Metabolites
Rats
Ischemia
Nuclear magnetic resonance
Phosphates
Cytosol
Phosphorus
Magnetic Resonance Spectroscopy
Adenosine Triphosphate
Metabolism
Nuclear magnetic resonance spectroscopy
Tissue
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

Mark Jeffrey, F., Storey, C. J., Nunnally, R. L., & Malloy, C. R. (1989). Effect of ischemia on NMR detection of phosphorylated metabolites in the intact rat heart. Biochemistry, 28(13), 5323-5326.

Effect of ischemia on NMR detection of phosphorylated metabolites in the intact rat heart. / Mark Jeffrey, F.; Storey, Charles J.; Nunnally, Ray L.; Malloy, Craig R.

In: Biochemistry, Vol. 28, No. 13, 1989, p. 5323-5326.

Research output: Contribution to journalArticle

Mark Jeffrey, F, Storey, CJ, Nunnally, RL & Malloy, CR 1989, 'Effect of ischemia on NMR detection of phosphorylated metabolites in the intact rat heart', Biochemistry, vol. 28, no. 13, pp. 5323-5326.
Mark Jeffrey, F. ; Storey, Charles J. ; Nunnally, Ray L. ; Malloy, Craig R. / Effect of ischemia on NMR detection of phosphorylated metabolites in the intact rat heart. In: Biochemistry. 1989 ; Vol. 28, No. 13. pp. 5323-5326.
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